alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis
INS 1100(iv)
Listed as a flour treatment agent by Codex.
Listed as flour treatment agents (Codex)
- Codex Alimentarius: Flour treatment agent — CXS 192-1995 Table Three, INS 1100(iv)
This alpha-amylase is made from a Bacillus megaterium gene expressed in Bacillus subtilis, a common arrangement in modern enzyme production. It breaks down starch and is used in baking and starch processing.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | No listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it. | ||
| Canada | No listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here. | ||
| European Union | No listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it. | ||
| Great Britain | No listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it. | ||
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | No listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance. | ||
| United States | Not listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited. | ||
| Codex Alimentarius | Permitted | no numerical limit (good manufacturing practice) | CXS 192-1995 Table Three, INS 1100(iv)retrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| Codex Alimentarius | — | 1 GMP |
|---|
No regulator sets a figure for alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis. That is not an absence of control: where the listing says quantum satis or good manufacturing practice, the quantity is limited to what the effect requires.
What it is made from
Produced by fermenting Bacillus subtilis carrying the amylase gene from Bacillus megaterium. The enzyme is purified away from the production organism.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis
Synonyms: Glycogenase
Assay, identification and purity criteria
Description and assay
- Specification history
- α -AMYLASE from BACILLUS MEGATERIUM expressed in BACILLUS SUBTILIS Prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999), superseding tentative specifications prepared at the 37th JECFA (1990), published in FNP 52 (1992). An ADI 'not specified' was established at the 37th JECFA (1990)
- SOURCES
- Produced by the controlled fermentation of Bacillus subtilis containing the gene for alpha-amylase from Bacillus megaterium . The strain of Bacillus subtilis is non-pathogenic and non-toxicogenic (for example ATCC 39,701). When fermentation is complete, the broth is clarified by centrifugation or filtration. The clarified broth containing the soluble enzyme is ultrafiltered to produce the desired activity.
- Active principles
- alpha-Amylase
- Systematic names and numbers
- 1,4-alpha-D-Glucan glucanohydrolase (EC 3.2.1.1)
- Reactions catalysed
- Endohydrolysis of 1,4-alpha-D-glucosidic linkages in polysaccharides, containing three or more 1,4-alpha-linked D-glucose units
- Secondary enzyme activities
- Glycosyl transferase, protease
- DESCRIPTION
- Typically tan to dark brown liquid containing the active enzyme
- FUNCTIONAL USES
- Enzyme preparation Used in starch hydrolysis
- GENERAL SPECIFICATIONS
- Must conform to the General Specifications for Enzyme Preparations Used in Food Processing (See Volume Introduction)
Identification
- alpha-Amylase activity (Vol. 4)
- The sample shows bacterial alpha-amylase activity
Every name it appears under
0 recorded names. A label may use any of them.
Hazard reference values
| Type | Value | Population | Assessed by | Opinion |
|---|---|---|---|---|
| ADI | not specified | — | JECFA | 1990 |
Evidence and how bodies read it
Studies, and the differing conclusions regulators drew from them, are curated rather than machine-read, and none has been written for this substance yet.
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is INS 1100(iv)?
- INS 1100(iv) is alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis. This alpha-amylase is made from a Bacillus megaterium gene expressed in Bacillus subtilis, a common arrangement in modern enzyme production. It breaks down starch and is used in baking and starch processing.
- What is alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis used for in food?
- It is listed as a flour treatment agent — the role Codex assigns it. This page shows every function each regulator records, with the citation for each.
- Where is alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis permitted?
- Of the seven regulators this site reads, the Codex standard permits it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis vegan?
- Yes. alpha-Amylase from Bacillus megaterium expressed in Bacillus subtilis is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- gsfa retrieved 2026-09-07
- jecfaspecs retrieved 2026-09-23
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