Carbohydrase from Bacillus licheniformis

INS 1100(vi)

Listed as a flour treatment agent and an enzyme by Codex and the US.

Listed as flour treatment agents (Codex) · enzymes (US)

This carbohydrase preparation breaks starch down into sugars, used in syrup manufacture, baking and brewing. The heat tolerance of this organism's enzymes is what makes them useful: they survive the temperatures at which starch actually gels.

Where it is and isn’t permitted

Status of Carbohydrase from Bacillus licheniformis by jurisdiction. Every jurisdiction this site covers is listed, including those where the substance does not appear.
JurisdictionStatusScope and limitsCitation
Australia and New ZealandFood Standards Australia New ZealandNo listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it.
CanadaHealth CanadaNo listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here.
European UnionEuropean Commission / EFSANo listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it.
Great BritainFood Standards Agency / Food Standards ScotlandNo listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it.
JapanConsumer Affairs AgencyNo listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance.
South KoreaMinistry of Food and Drug SafetyNo listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance.
United StatesFood and Drug AdministrationNot listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited.
Codex AlimentariusCodex Alimentarius Commission / JECFAPermitted, with conditions

1 food category

no numerical limit (good manufacturing practice)

Note XS152 Excluding products conforming to the Standard for Wheat Flour (CXS 152-1985).

CXS 192-1995 Table Three, INS 1100(vi)and 1 further provisionretrieved 2026-09-07

How much is permitted

A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.

Codex Alimentarius—2 GMP

No regulator sets a figure for Carbohydrase from Bacillus licheniformis. That is not an absence of control: where the listing says quantum satis or good manufacturing practice, the quantity is limited to what the effect requires.

What FDA’s inventory records

This page holds no listing for Carbohydrase from Bacillus licheniformis in the parts of 21 CFR this site reads. FDA’s Substances Added to Food inventory does carry it, as CARBOHYDRASE FROM BACILLUS LICHENIFORMIS, pointing at no regulation at all.

Technical effects FDA records: enzyme.

The inventory is an information resource: a row means FDA holds information about the substance’s use in food, not that the substance is listed for that use. It is shown apart from the permissions above and is not counted as a US permission anywhere on this site.

What it is made from

Produced by culturing Bacillus licheniformis and recovering the enzyme, which is then removed or inactivated.

  • Vegan
  • Vegetarian

Compare other additives on the dietary indexes.

How JECFA defines it

The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.

INS 1100 Carbohydrase from Bacillus licheniformis

Synonyms: Diastase; Glycogenase; Ptyalin

Assay, identification and purity criteria

Description and assay

Specification history
Prepared at the 29th JECFA (1986), published in FNP 34 (1986) and in FNP 52 (1992) An ADI 'not specified' was established at the 29th JECFA (1985)
SYNONYMS SOURCES
Diastase, ptyalin, glycogenase; INS No.1100 Commercial enzyme preparations are produced by the controlled fermentation of Bacillus licheniformis .
Active principles Systematic names and numbers Reactions catalyzed
alpha-Amylase 1,4-alpha-D-Glucan glucanohydrolase (EC 3.2.1.1) The enzyme preparations hydrolyze 1,4-alpha-glucosidic linkages in polysaccharides, yielding dextrins and oligo- and monosaccharides.
Secondary enzyme activities DESCRIPTION
Microbial serine proteinase (EC 3.4.21.14) Off-white to tan amorphous powders or as brown liquids; soluble in water, but practically insoluble in ethanol, chloroform and ether
FUNCTIONAL USES
Enzyme preparation Used in the preparation of and/or in cereals and starch, fruits and vegetables, beverages, sugar and honey, confectionery and bakery.
GENERAL SPECIFICATIONS Alpha-Amylase activity (Vol. 4)
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (see Volume Introduction) The sample shows bacterial alpha-amylase activity

FAO JECFA Monograph 1 (2006)

Every name it appears under

2 recorded names. A label may use any of them.

INS number

  • 1100(vi)

Other names

  • CARBOHYDRASE FROM BACILLUS LICHENIFORMIS

Hazard reference values

No reference value for this substance in the sources we hold.

Evidence and how bodies read it

Studies, and the differing conclusions regulators drew from them, are curated rather than machine-read, and none has been written for this substance yet.

Common questions

Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.

What is INS 1100(vi)?
INS 1100(vi) is Carbohydrase from Bacillus licheniformis. This carbohydrase preparation breaks starch down into sugars, used in syrup manufacture, baking and brewing. The heat tolerance of this organism's enzymes is what makes them useful: they survive the temperatures at which starch actually gels.
What is Carbohydrase from Bacillus licheniformis used for in food?
It is listed as a flour treatment agent and an enzyme — the role Codex and the US assign it. This page shows every function each regulator records, with the citation for each.
Where is Carbohydrase from Bacillus licheniformis permitted?
Of the seven regulators this site reads, the Codex standard permits it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
Is Carbohydrase from Bacillus licheniformis vegan?
Yes. Carbohydrase from Bacillus licheniformis is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.

Sources for this page

  • gsfa retrieved 2026-09-07
  • jecfaspecs retrieved 2026-09-23

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