Carotenal, beta-apo-8'-
1107-26-2 · 12676-20-9 · -po-8'-arotenal · .beta.-apo-8'-Carotenal · 4-07-00-01782 (Beilstein Handbook Reference) · 8'-APO-.BETA.,.PSI.-CAROTENAL · 8'-apo-.beta.-Caroten-8'-al · 8'-APO-.BETA.-CAROTENAL · 8'-apo-beta,psi-caroten-8'-al · 8'-Apo-beta,psi-carotenal · 8'-Apo-beta-caroten-8'-al · 8'-Apo-beta-carotenal · 8'-Apo-beta-carotenal, all-trans- · 8'-Apoaldehyde · 8'Apo-beta,psi-carotenal · all-trans-8'-apo-beta-carotenal · all-trans-beta-Apo-8'-carotenal · Apocarotenal · Apocarotenal, United States Pharmacopeia (USP) Reference Standard · beta-apo-8'-carotenal · Beta-apo-8'-carotenal (C 30) · beta-Apo-8'-carotenal (C30) · BETA-APO-8'-CAROTENAL (TRANS) · beta-apo-Carotenal · beta-Apocarotenal · BRN 2064131 · C Orange 16 · C.I. Food Orange 6 · Carotenal, beta-apo-8'- · CCRIS 7933 · CI Food Orange 6 · E 160e · Food orange 6 · INS NO.160E · INS-160E · trans-8'-Apo-?-Caroten-8'-al · trans-?-Apo-8?-carotenal · trans-beta-Apo-8'-carotenal · trans-beta-Apo-8'-carotenal, >=96.0% (UV) · trans-|A-Apo-8 inverted exclamation marka-carotenal · |?-Apo-8'-carotenal · |_-Apo-8 inverted exclamation marka-carotenal · |A- Apo- 8'- carotenal · |A-?Apo-?8'-?carotenal (Standard) · E160e · 160e · (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohe x-1-enyl)heptadeca-2,4,6,8,10,12,14,16-octaenal · (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohex-1-en-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal · (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohexen-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal · 2,4,6,8,10,12,14,16-Heptadecaoctaenal, 2,6,11,15-tetramethyl-17-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (2E,4E,6E,8E,10E,12E,14E,16E)- · 2,4,6,8,10,12,14,16-Heptadecaoctaenal, 2,6,11,15-tetramethyl-17-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (all-E)- · 2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohex-1-en-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal · Beta-apo-8'-carotenal · Beta-apo-8'-carotenal (C 30) · C 30 · BEETA-APO-8′-KAROTENAAL (C30) · Beta-apo-8'- karotenal (C 30) · Beta-apo-8'-carotenale (C30) · Beta-apo-8'-karotenal (C 30) · Beta-apo-8'-karotenalis (C 30) · Beta-apo-8’-karotenaali (C30) · Beta-apo-8’-karotenal (C 30) · beta-apo-8’-karotenal (C30) · Beta-apo-8’-karotināls (C 30) · Beta-apo-8′-carotenal (C 30) · Beta-apo-8′-carotenal (C30) · Beta-apo-8′-Carotinal (C 30) · BETA-APO-8′-KAROTENAALI (C30) · BETA-APO-8′-KAROTENAL (C30) · BETA-APO-8′-KAROTENALIS (C30) · béta-apo-8'-Karotinal (C30) · Β-ΑΠΟ-8′-ΚΑΡΟΤΕΝΑΛΗ (C30) · ΒETA-APO-8′-KAROTENĀLS (C30) · β-APO-8′-CAROTÉNAL (C30) · β-apo-8′-karotenaal (C30) · β-APO-8′-KAROTENAL (C 30) · β-apo-8′-karotenal (C30) · β-apocaroténal-8' (C 30) · β-απο-8’-καροτενάλη (C 30) · БЕТА-АПО-8′-КАРОТЕНАЛ (C30) · Бета-апо-8′-каротенал (С 30)E number E160eINS 160eCAS 1107-26-2
Listed as a colour by Codex, the EU and the US.
Listed as colours (EU, US, Codex)
- European Union: Colours — Regulation (EC) No 1333/2008, Annex II, Part B, 1. Colours (consolidated text 02008R1333-20260818)
- United States: Color additive (21 CFR part 73) — 21 CFR 73.90
- United States: Color or coloring adjunct — FDA, Substances Added to Food: BETA-APO-8'-CAROTENAL
- Codex Alimentarius: Colour — CXS 192-1995 Table One, INS 160e

Beta-apo-8'-carotenal is a synthetic carotenoid giving an orange to orange-red colour, used in drinks, dairy products and sauces. It is chemically related to beta-carotene but gives a redder shade.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | Permitted, with conditions | 48 food categories no numerical limit (good manufacturing practice) Show every food category
| Food Standards Code, Schedule 15, S15—5and 47 further provisionsretrieved 2026-10-17 |
| Canada | No listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here. | ||
| European Union | Permitted, with conditions | 27 food categories highest permitted level 500 mg/l Show every food category
| Regulation (EC) No 1333/2008, Annex IIand 26 further provisionsretrieved 2026-10-18 |
| Great Britain | Permitted, with conditions | 36 food categories highest permitted level 500 mg/kg or mg/l Show every food category
| Assimilated Regulation (EC) No 1333/2008and 35 further provisionsretrieved 2026-10-18 |
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | Permitted, with conditions | Designated food additive: Standards and Specifications for Food Additives, Part III, 1. …Designated food additive: Standards and Specifications for Food Additives, Part III, 1. 일반식품첨가물 (general food additives), β-아포-8'-카로티날 (“β-Apo-8'-Carotenal”, the English the Code prints beside it). Main use (주용도) of β-아포-8'-카로티날: 착색료. Standard for use (사용기준) of β-아포-8'-카로티날: (1) [must not be used in the foods listed] “β-아포-8'-카로티날은 아래의 식품에 사용하여서는 아니 된다. 1. 천연식품[식육류, 어패류, 과일류, 채소류, 해조류, 콩류 등 및 그 단순 가공품(탈피, 절단 등)] 2. 다류 3. 커피 4. 고춧가루, 실고추 5. 김치류 6. 고추장, 조미고추장 7. 식초” The Korean is the standard as published. There is no official English translation of the current Code (the English version on MFDS’s website is the 2020 edition and has no legal effect); the English in square brackets is this site’s description of what each sentence does, not a translation of the foods or the levels. Compositional specifications (Part IV) are not imported, nor are the general standards in Part II, which apply to every additive. | Standards and Specifications for Food Additives (식품첨가물의 기준 및 규격), MFDS Notification, Part III (Standards for use, by item)retrieved 2026-10-18 |
| United States | Permitted | 21 CFR 73.90 lists the colour additive β-apo-8′-carotenal for food use under part 73. The section is also held on this site as the page apo-8-carotenal; read it for the specifications. | 21 CFR 73.90retrieved 2026-10-18 |
| Codex Alimentarius | Permitted, with conditions | 30 food categories highest permitted level 2,000 mg/kg Show every food category and its notes
| CXS 192-1995 Table One, INS 160eand 29 further provisionsretrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| Australia and New Zealand | — | 48 GMP |
|---|---|---|
| European Union | 50 mg/kg – 500 mg/kg | 26 numeric limit, 1 quantum satis |
| Great Britain | 50 mg/kg – 500 mg/kg | 30 numeric limit, 5 several limits, 1 quantum satis |
| South Korea | — | 1 no quantity stated |
| United States | — | 1 no quantity stated |
| Codex Alimentarius | 1 mg/kg – 2,000 mg/kg | 30 numeric limit |
What it is made from
Produced by chemical synthesis. Although the same molecule occurs in small amounts in citrus and spinach, the additive is not extracted from them.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
- CAS registry number
- 1107-26-2
- InChIKey
- DFMMVLFMMAQXHZ-DOKBYWHISA-N
- PubChem
- CID 5478003
The two specifications, compared
The European Union sets identity and purity by regulation; JECFA publishes its own through FAO, advisory until a country adopts it. Both documents are below in full. These are the parameters each puts a number on.
| Parameter | European Union | JECFA |
|---|---|---|
| Assay | no less than 96 % | no less than 96 % |
| Sulphated ash | no more than 0.1 % | no more than 0.1 % |
| Lead | no more than 2 mg/kg | no more than 2 mg/kg |
| Arsenic | no more than 3 mg/kg | — |
| Mercury | no more than 1 mg/kg | — |
| Cadmium | no more than 1 mg/kg | — |
The two documents state the same figures for every parameter compared here.
How the EU defines it
The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.
E 160e Beta-apo-8′-carotenal (c30)
These specifications apply predominantly to the all-trans isomer of β-apo-8′-carotenal together with minor amounts of other carotenoids. Diluted and stabilised forms are prepared from β-apo-8′-carotenal meeting these specifications and include solutions or suspensions of ß-apo-8′carotenal in edible fats or oils, emulsions and water dispersible powders. These preparations may have different cis/trans isomer ratios.
Synonyms: CI Food Orange 6
Assay, identification and purity criteria
Description and assay
- Colour Index No
- 40820
- Einecs
- 214-171-6
- Chemical name
- β-Apo-8′-carotenal; trans-β-Apo-8′carotene-aldehyde
- Chemical formula
- C₃₀H₄₀O
- Molecular weight
- 416,65
- Assay
- Not less than 96 % of total colouring matters [image] 2 640 at 460-462 nm in cyclohexane
- Description
- Dark violet crystals with metallic lustre or crystalline powder
Identification
- Spectrometry
- Maximum in cyclohexane at 460-462 nm
Purity criteria
- Sulphated ash
- Not more than 0,1 %
- Subsidiary colouring matters
- Carotenoids other than β-apo-8′-carotenal: not more than 3,0 % of total colouring matters
- Arsenic
- Not more than 3 mg/kg
- Lead
- Not more than 2 mg/kg
- Mercury
- Not more than 1 mg/kg
- Cadmium
- Not more than 1 mg/kg
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
INS 160e beta-Apo-8'-carotenal
These specifications apply to β-apo-8’-carotenal which consists predominantly of all-trans- β-apo-8’-carotenal and may also contain minor quantities of other carotenoids such as all-trans-crocetindialdehyde, all ‐ trans ‐ β ‐ apo ‐ 12’ ‐ carotenal and all ‐ trans-β ‐ carotene. Commercial preparations of β-apo-8’-carotenal intended for use in food are prepared from β-apo-8’-carotenal meeting these specifications and are formulated as suspensions in edible oil, emulsions and water dispersible powders. These preparations may also contain cis isomers.
Synonyms: CI Food Orange 6; CI (1975) No. 40820; INS No. 160e
Assay, identification and purity criteria
Description and assay
- Specification history
- β -apo-8’-CAROTENAL Prepared at the 87 th JECFA (2019) and published in FAO Monographs 23 (2019), superseding specifications prepared at the 74thJECFA (2011) and published in FAO Monographs 11 (2011). A group ADI of 0-5 mg/kg bw expressed as the sum of carotenoids including β-carotene, β-apo-8’-carotenal, and the methyl and ethyl esters of β-apo-8’-carotenoic acid was established at the 18 th JECFA (1974).
- Chemical names
- ß-Apo-8’-carotenal, 8’-apo-ß-carotene-al 2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethyl-1-cyclohexenyl)heptadeca-2,4,6,8,10,12,14,16-octaenal
- C.A.S. number
- 1107-26-2
- Chemical formula
- C 40 H 40 O
- Structural formula
- All- trans -β-apo-8’-carotenal (main compound)
- Formula weight
- 416.65
- Assay
- Not less than 96% total colouring matters
- DESCRIPTION
- Deep violet crystals with metallic lustre or crystalline powder; sensitive to oxygen and light and should therefore be kept in a light-resistant container under inert gas.
- FUNCTIONAL USES
- Colour
- METHOD OF ASSAY
- Total colouring matters content by spectrophotometry Proceed as directed under Total Colouring Matters Content – Colouring Matters Content by Spectrophotometry, Procedure 2, using the following conditions: Sample weight (W): 0.08 g (±0.01 g); Volume of the three volumetric flasks: V 1 = V 2 = V 3 = 100 ml; Volume of the two pipets: v 1 = v 2 = 5 ml; Specific absorbance of the standard: A 1% = 2640 ; 1cm Wavelength of maximum absorption: λ max about 461 nm. Calculation Calculate the percentage of total colouring matters using the following formula: A × V 1 × D Total colouring matters (%, w/w) = 1% A 1cm × W where A is the absorbance of the twice-diluted sample solution at 461 nm; and D is the dilution factor (V 2 xV 3 )/(v 1 /v 2 ).
Identification
- Solubility (Vol. 4)
- Insoluble in water; slightly soluble in ethanol; sparingly soluble in vegetable oils.
- Spectrophotometry (Vol. 4)
- Determine the absorbance of the diluted sample solution used in the Method of Assay at 460 nm and 488 nm. Determine the absorbance at 332 nm of a solution containing a ten-fold higher concentration as that of the diluted sample solution used in the Method of Assay. The ratio A 488 /A 460 is between 0.77 and 0.85. The ratio of A 332 /A 460 is between 0.63 and 0.75.
Purity criteria
- Sulfated ash (Vol. 4)
- Not more than 0.1% Test 2 g of the sample (Method I)
- Carotenoids other than ß-apo-8’- carotenal
- Not more than 3% of total colouring matters. See description under TESTS
- Lead (Vol. 4)
- Not more than 2 mg/kg. Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”).
- Test: Carotenoids other than ß-apo-8’- carotenal
- Chromatographic system − HPLC equipped with a UV/Vis detector or a photodiode array detector, refrigerated auto sampler and integrator − Detector wavelength: 463 nm − Column: Reverse phase C18; Suplex pkb-100 (250 x 4.6 mm, 5 μm) from Supelco or equivalent − Mobile phase: In a 1000 ml volumetric flask, dissolve 50 mg BHT in 20 ml 2 ‐ propanol and add 0.2 ml N ‐ ethyldiisopropyl ‐ amine, 25 ml 0.2% aqueous ammonium acetate solution, 455 ml acetonitrile, and 3pprox.. 450 ml methanol. Mixture cools and contracts. Allow to reach room temperature and dilute to volume with methanol. Discard after 2 days. − Isocratic elution − Column temperature: 30° − Flow rate: 0.6 ml/min − Injection volume: 10 μl − Temperature of the autosampler: (3pprox.. 15°) − Run time: 4pprox.. 35 min Reagents - Butylated hydroxytoluene (BHT), reagent grade - 2-Propanol, HPLC grade - N-ethyldiisopropyl-amine, reagent grade - Ammonium acetate, reagent grade - Acetonitrile, HPLC grade - Methanol, HPLC grade - Ethanol, HPLC grade - Tetrahydrofuran, HPLC grade Sample solution Weigh accurately (to ±0.1 mg) 0.010 g of the sample and dissolve in tetrahydrofuran (stabilized with 0.025% BHT). Transfer to a 100 ml volumetric flask and bring to volume with tetrahydrofuran. Dilute to the ratio of 1:10 with ethanol. Procedure Inject the sample solution using the conditions detailed under Chromatographic system . The retention times for all- trans -β- apo-8’-carotenal is in the range of 7-9 min and corresponds to the largest peak in the chromatogram. The relative retention times of minor carotenoids with respect to the retention time of all- trans -β-apo-8’-carotenal are: all- trans-crocetindialdehyde (0.54); all ‐ trans ‐ β ‐ apo ‐ 12’ ‐ carotenal (0.84); all ‐ trans -β ‐ carotene (2.55). Integrate the areas of the peaks in the chromatogram. Calculation Calculate the percentage of carotenoids other than β-apo-8’- carotenal (%, w/w) using the following formula: w Carotenoids other than β − apo − 8’ − carotenal (%, ) w A total – A β −apo−8’−carotenal = A total where A total is the sum of the area of all the peaks in the chromatogram, excluding the solvent peak (area units); and A β-apo-8’-carotenal is the area of the peak of β-apo-8’-carotenal in the chromatogram (area units).
Every name it appears under
81 recorded names. A label may use any of them.
E number
- E160e
INS number
- 160e
CAS registry number
- 1107-26-2
- 12676-20-9
As it appears in regulation
- Beta-apo-8'-carotenal
- Beta-apo-8'-carotenal (C 30)
- C 30
Other names
- -po-8'-arotenal
- .beta.-apo-8'-Carotenal
- 4-07-00-01782 (Beilstein Handbook Reference)
- 8'-APO-.BETA.,.PSI.-CAROTENAL
- 8'-apo-.beta.-Caroten-8'-al
- 8'-APO-.BETA.-CAROTENAL
- 8'-apo-beta,psi-caroten-8'-al
- 8'-Apo-beta,psi-carotenal
- 8'-Apo-beta-caroten-8'-al
- 8'-Apo-beta-carotenal
- 8'-Apo-beta-carotenal, all-trans-
- 8'-Apoaldehyde
- 8'Apo-beta,psi-carotenal
- all-trans-8'-apo-beta-carotenal
- all-trans-beta-Apo-8'-carotenal
- Apocarotenal
- Apocarotenal, United States Pharmacopeia (USP) Reference Standard
- beta-apo-8'-carotenal
- Beta-apo-8'-carotenal (C 30)
- beta-Apo-8'-carotenal (C30)
- BETA-APO-8'-CAROTENAL (TRANS)
- beta-apo-Carotenal
- beta-Apocarotenal
- BRN 2064131
- C Orange 16
- C.I. Food Orange 6
- Carotenal, beta-apo-8'-
- CCRIS 7933
- CI Food Orange 6
- E 160e
- Food orange 6
- INS NO.160E
- INS-160E
- trans-8'-Apo-?-Caroten-8'-al
- trans-?-Apo-8?-carotenal
- trans-beta-Apo-8'-carotenal
- trans-beta-Apo-8'-carotenal, >=96.0% (UV)
- trans-|A-Apo-8 inverted exclamation marka-carotenal
- |?-Apo-8'-carotenal
- |_-Apo-8 inverted exclamation marka-carotenal
- |A- Apo- 8'- carotenal
- |A-?Apo-?8'-?carotenal (Standard)
Systematic names
- (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohe x-1-enyl)heptadeca-2,4,6,8,10,12,14,16-octaenal
- (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohex-1-en-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal
- (2E,4E,6E,8E,10E,12E,14E,16E)-2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohexen-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal
- 2,4,6,8,10,12,14,16-Heptadecaoctaenal, 2,6,11,15-tetramethyl-17-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (2E,4E,6E,8E,10E,12E,14E,16E)-
- 2,4,6,8,10,12,14,16-Heptadecaoctaenal, 2,6,11,15-tetramethyl-17-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (all-E)-
- 2,6,11,15-tetramethyl-17-(2,6,6-trimethylcyclohex-1-en-1-yl)heptadeca-2,4,6,8,10,12,14,16-octaenal
The EU re-evaluation programme
Every additive authorised in the EU before 20 January 2009 was put back through EFSA by Commission Regulation (EU) No 257/2010, and this one is named in it. What the Regulation required is below; whether the opinion has since been published is not something this site records.
- To be evaluated by 31 December 2015. Named in Annex II, Part I, point 3 as “Beta-apo-8’-carotenal (C30)”, among the additives given priority within its class.
Hazard reference values
| Type | Value | Population | Assessed by | Opinion |
|---|---|---|---|---|
| ADI | 0.3 mg/kg bw/day | — | JECFA | 2019 |
Evidence and how bodies read it
What assessing bodies concluded
Each conclusion is paraphrased in the body's own framing, from the published opinion linked beneath it. Where bodies differ, both stand; this site does not adjudicate between them or add a view of its own.
EFSA 2014
After a re-examination of the kidney slides from the 13-week rat study underlying its 2012 ADI, the ANS Panel revised the ADI for β-apo-8'-carotenal (E 160e) from 0.05 to 0.3 mg/kg bw/day. It concluded that the reported uses and use levels, based on a limited number of food categories, would not be of safety concern.
EFSA 2012
The ANS Panel concluded that β-apo-8'-carotenal (E 160e) raised no genotoxicity concern and, based on kidney findings in a 13-week rat study, established an ADI of 0.05 mg/kg bw/day (the SCF had withdrawn the earlier ADI of 5 mg/kg bw/day in 2000). Refined exposure estimates reached this ADI for adults on average and exceeded it for high-consuming adults and for children.
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is E 160e?
- E 160e is Carotenal, beta-apo-8'-. Beta-apo-8'-carotenal is a synthetic carotenoid giving an orange to orange-red colour, used in drinks, dairy products and sauces. It is chemically related to beta-carotene but gives a redder shade.
- What is Carotenal, beta-apo-8'- used for in food?
- It is listed as a colour — the role Codex, the EU and the US assign it. This page shows every function each regulator records, with the citation for each.
- Where is Carotenal, beta-apo-8'- permitted?
- Of the seven regulators this site reads, Australia and New Zealand, the EU, Great Britain, South Korea, the US and the Codex standard permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is Carotenal, beta-apo-8'- vegan?
- Yes. Carotenal, beta-apo-8'- is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- anz retrieved 2026-10-17
- eu retrieved 2026-10-18
- gb retrieved 2026-10-18
- kr retrieved 2026-10-18
- us-listings retrieved 2026-10-18
- gsfa retrieved 2026-09-07
- euspecs retrieved 2026-10-18
- jecfaspecs retrieved 2026-09-23
Full licence terms and publisher details on Sources. How this page is built and updated: Method.
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