Quinoline yellow
8004-92-0 · E 104 · QUINOLINE YELLOW · E104 · 104 · Disodium 2-(1,3-dioxoinden-2-yl)quinoline-6,8-disulfonate · Quinoline Yellow · Amarelo de quinoleína · Amarillo de quinoleína · Chinolinegeel · Chinolingelb · Chinolino geltonasis · Chinolinová žluť · Chinolínová žltá · Galben de chinolină · GIALLO CHINOLINA · Giallo di chinolina · Hinolīna dzeltenais · ISFAR TAL-KINOLIN · Isfar tal-Kinolina · Jaune de quinoléine · Kinoliinikeltainen · Kinoliinkollane · Kinolingult · kinolinsko rumeno · Kinolinsárga · Quinoline Yellow · Quinolingult · Żółcień chinolinowa · ΚΙΤΡΙΝΟ ΚΙΝΟΛΙΝΗΣ · Κίτρινο της κινολίνης · Хинолин жълтоE number E104INS 104CAS 8004-92-0
Listed as a colour by Codex and the EU.
Listed as colours (EU, Codex)
- European Union: Colours — Regulation (EC) No 1333/2008, Annex II, Part B, 1. Colours (consolidated text 02008R1333-20260818)
- Codex Alimentarius: Colour — CXS 192-1995 Table One, INS 104
Quinoline Yellow is a synthetic greenish-yellow dye used in soft drinks, confectionery and desserts. It is one of the six colours that must carry the activity-and-attention warning in the EU.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | Permitted, with conditions | 44 food categories Show every food category
| Food Standards Code, Schedule 15, S15—5and 43 further provisionsretrieved 2026-10-17 |
| Canada | No listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here. | ||
| European Union | Permitted, with conditions | 29 food categories highest permitted level 200 mg/l Show every food category
| Regulation (EC) No 1333/2008and 28 further provisionsretrieved 2026-10-18 |
| Great Britain | Permitted, with conditions | 27 food categories highest permitted level 200 mg/kg or mg/l Show every food category
| Assimilated Regulation (EC) No 1333/2008and 26 further provisionsretrieved 2026-10-18 |
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | No listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance. | ||
| United States | Not listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited. | ||
| Codex Alimentarius | Permitted, with conditions | 31 food categories highest permitted level 500 mg/kg Show every food category and its notes
| CXS 192-1995 Table One, INS 104and 30 further provisionsretrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| Australia and New Zealand | — | 44 no quantity stated |
|---|---|---|
| European Union | 10 mg/kg – 200 mg/kg | 29 numeric limit |
| Great Britain | 10 mg/kg – 200 mg/kg | 24 numeric limit, 3 several limits |
| Codex Alimentarius | 9 mg/kg – 500 mg/kg | 31 numeric limit |
What a label must say
Words a food containing Quinoline yellow is obliged to carry, quoted from the instrument that imposes them. A required statement is a fact about the regulation, not a finding about the substance — every rule below applies where the additive is authorised.
‘name or E number of the colour(s)’: may have an adverse effect on activity and attention in children.
A food containing any of six colours must carry this statement. Regulation (EC) No 1333/2008, Article 24 and Annex V · Assimilated Regulation (EC) No 1333/2008, Annex V
Except: Foods where the colour was used for health or other marking on meat products, or for stamping or decorative colouring on eggshells. Beverages containing more than 1.2 % alcohol by volume.
Every rule collected at what a label must say.
What it is made from
A mixture of the sulfonated forms of 2-(2-quinolyl)indan-1,3-dione, synthesised from petroleum-derived aromatic intermediates. No animal material is involved.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
- CAS registry number
- 8004-92-0
The two specifications, compared
The European Union sets identity and purity by regulation; JECFA publishes its own through FAO, advisory until a country adopts it. Both documents are below in full. These are the parameters each puts a number on.
| Parameter | European Union | JECFA |
|---|---|---|
| Assay | no less than 70 % | no less than 70 % |
| Lead | no more than 2 mg/kg | no more than 2 mg/kg |
| Arsenic | no more than 3 mg/kg | — |
| Mercury | no more than 1 mg/kg | — |
| Cadmium | no more than 1 mg/kg | — |
The two documents state the same figures for every parameter compared here.
How the EU defines it
The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.
E 104 Quinoline yellow
Quinoline Yellow is prepared by sulfonating 2-(2-quinolyl) indan-1,3-dione or a mixture containing about two thirds 2-(2-quinolyl)indane-1,3-dione and one third 2-(2-(6-methylquinolyl))indane-1,3-dione. Quinoline Yellow consists essentially of sodium salts of a mixture of disulfonates (principally), monosulfonates and trisulfonates of the above compound and subsidiary colouring matters together with sodium chloride and/or sodium sulphate as the principal uncoloured components. Quinoline Yellow is described as the sodium salt. The calcium and the potassium salt are also permitted.
Synonyms: CI Food Yellow 13
Assay, identification and purity criteria
Description and assay
- Colour Index No
- 47005
- Einecs
- 305-897-5
- Chemical name
- The disodium salts of the disulfonates of 2-(2-quinolyl) indan-1,3-dione (principal component)
- Chemical formula
- C₁₈H₉N Na₂O₈S₂ (principal component)
- Molecular weight
- 477,38 (principal component)
- Assay
- Content not less than 70 % total colouring matters calculated as the sodium salt Quinoline Yellow shall have the following composition: Of the total colouring matters present: — not less than 80 % shall be disodium 2-(2-quinolyl) indan-1,3-dione-disulfonates — not more than 15 % shall be sodium 2-(2-quinolyl) indan-1,3-dione-monosulfonates — not more than 7,0 % shall be trisodium 2-(2-quinolyl) indan-1,3-dione-trisulfonate [image] 865 (principal component) at ca. 411 nm in aqueous acetic acid solution
- Description
- Yellow powder or granules
- Appearance of the aqueous solution
- Yellow
Identification
- Spectrometry
- Maximum in aqueous acetic acid solution of pH 5 at ca. 411 nm
Purity criteria
- Water insoluble matter
- Not more than 0,2 %
- Subsidiary colouring matters
- Not more than 4,0 %
- 2-methylquinoline
- [image] | Total not more than 0,5 % 2-methylquinoline-sulfonic acid Phthalic acid 2,6-dimethyl quinoline 2,6-dimethyl quinoline sulfonic acid
- 2-(2-quinolyl)indan-1,3-dione
- Not more than 4 mg/kg
- Unsulfonated primary aromatic amines
- Not more than 0,01 % (calculated as aniline)
- Ether extractable matter
- Not more than 0,2 % under neutral conditions
- Arsenic
- Not more than 3 mg/kg
- Lead
- Not more than 2 mg/kg
- Mercury
- Not more than 1 mg/kg
- Cadmium
- Not more than 1 mg/kg
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
INS 104 Quinoline Yellow
Quinoline Yellow is manufactured by sulfonating 2-(2-quinolyl)-1,3-indandione. It consists predominantly of sodium salts of disulfonates of 2-(2-quinolyl)-1,3-indandione with smaller amounts of monosulfonates and trisulfonates; and subsidiary colouring matters, sodium chloride and/or sodium sulfate. Quinoline Yellow may be converted to the corresponding aluminium lake, in which case only the General Specifications for Aluminium Lakes of Colouring Matters apply.
Synonyms: INS No. 104; CI Food Yellow 13; CI (1982) No. 47005 CI (1975) No. 47005
Assay, identification and purity criteria
Description and assay
- Specification history
- Prepared at the 82 nd JECFA (2016) and published in FAO JECFA Monograph 19 (2016), superseding tentative specifications prepared at the 74 th JECFA (2011) and published in FAO JECFA Monographs 11 (2011). An ADI of ADI of 0–3 mg/kg bw was established at the 82nd JECFA (2016).
- Chemical name
- Disodium 2-(2-quinolyl)indan-1,3-dionedisulphonate (principal component)
- C.A.S. number
- 80583-08-0 (principal component)
- Chemical formula
- C18H9NO8S2Na2 (principal component)
- Structural formula
- (Principal component) (NaO3S)2
- Formula weight
- 477.38 (Principal component)
- Assay
- Not less than 70% total colouring matters. Of the total colouring matters present: - not less than 80% of disodium 2-(2-quinolyl)-indan-1,3-dione-disulfonates; - not more than 15% of sodium 2-(2-quinolyl)-indan-1,3-dione-monosulfonates; - not more than 7% of trisodium 2-(2-quinolyl)-indan-1,3-dione-trisulfonate
- DESCRIPTION
- Yellow powder or granules
- FUNCTIONAL USES
- Colour
- METHOD OF ASSAY
- Determine total colouring matters using Procedure 1 in Volume 4 (under “Specific Methods, Food Colours”) and mono-, di- and trisulfonates of 2-(2-quinolyl)-indan-1,3-dione by reversed-phase HPLC (Vol.4). Total colouring matters Solvent: Water Absorptivity (a) = 8 7.9 L/(g∙cm) Wavelength of maximum absorbance = 414 nm Mono-, di- and trisulfonates Determine sodium salts of mono-, di- and trisulfonates of 2-(2-quinolyl)- indan-1,3-dione by reversed-phase HPLC using the following conditions: Solvent A: 0.1 M ammonium acetate in water/methanol (95:5, v/v) Solvent B: methanol Injection volume: 20 μl Column: Hypersil RP C8 (250 mm x 4.6 mm i.d.,5 μm particle size) or equivalent. Column temperature: 25 º Detector: UV-Vis/PDA at 414 nm Flow rate: 1 ml/min Gradient: Min %A %B 0 90 10 30 0 100 Standard solution (for retention times): 10 mg of Quinoline Yellow containing mono-, di-, and trisulfonates in 100 ml of water/methanol (75:25). Sample solution: 10 mg of sample in 100 ml of water/methanol (75:25). Calculation: Express the results as percentage of the peak area of each components/the peak area of total peaks on the chromatogram.
Identification
- Solubility (Vol. 4)
- Freely soluble in water; sparingly soluble in ethanol
- Spectrophotometry (Vol.4)
- The UV-visible absorption spectrum of an aqueous solution of the sample shows a maximum wavelength approximately at 414 nm.
Purity criteria
- Loss on drying at 135 o ,
- Not more than 30%
- chloride and sulfate as sodium salts (Vol. 4)
- Determine according to Loss on drying in Volume 4 (under “Specific Methods, Food Colours”).
- Water-insoluble matter (Vol. 4)
- Not more than 0.2%
- Subsidiary colouring matters
- Not more than 4 mg/kg of 2-(2-quinolyl)-1,3-indandione and 2-[2-(6-methylquinolyl)]-1,3-indandione See description under TESTS
- Organic compounds other than colouring matters (Vol. 4)
- Not more than 0.5%, sum of 2-methylquinoline, 2-methylquinolinesulfonic acid and phthalic acid See description under TESTS
- Unsulfonated primary aromatic amines (Vol. 4)
- Not more than 0.01% calculated as aniline
- Ether-extractable matter (Vol. 4)
- Not more than 0.2%
- Lead (Vol. 4)
- Not more than 2 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4 (under “General Methods, Metallic Impurities”).
- Zinc (Vol. 4)
- Not more than 50 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4 (under “General Methods, Metallic Impurities”).
- Test: Subsidiary colouring matters
- Determine 2-(2-quinolyl)-1,3-indandione and 2-[2-(6-methyl-quinolyl)]- 1,3-indandione by reversed-phase HPLC (Vol.4) using the following conditions: Solvent A: 0.05 M ammonium acetate Solvent B; acetonitrile Injection volume: 100 μl Column: C18 (250 mm x 4.6 mm i.d., 5 μm particle size) Detector: UV-Vis/PDA at 436 nm Flow rate: 1 ml/min Gradient: Min %A %B 0 40 60 19.9 40 60 20.0 0 100 35.0 0 100 Standards: 2-(2-quinolyl)-1,3-indandione, Sigma 01354 or equivalent; 2-[2-(6-methyl-quinolyl)]-1,3-indandione, Chemos GmbH or equivalent. Sample preparation: Dissolve 1 g of sample in 10 ml of hot water. Allow solution to cool to room temperature. Extract analytes using chloroform, evaporate the solvent and dissolve the residue in acetonitrile.
- Test: Organic compounds other than colouring matters (Vol. 4)
- Determine sum of 2-methylquinoline, 2-methylquinolinesulfonic acid and phthalic acid by reversed-phase HPLC (under “Specific Methods, Food Colours”) using the following conditions: Solvent A: 0.2 M ammonium acetate in water/methanol (95:5, v/v) Solvent B: methanol I njection volume: 20 μ l Detector wavelength: UV/PDA at 254 nm Flow rate: 1 ml/min Gradient: Min %A %B 0 100 0 8.8 80 20 9.0 0 100 12.0 0 100 Note: A general gradient for the separation of organic compounds other than colouring matters in several food colours is given in Vol. 4. The above gradient may be use for the analytes in Quinoline Yellow. Standard solution: 0.1% of quinaldine-6-sulfonic acid, quinaldine-6,8-disulfonic acid, trisodium salt of 4-sulfophthalic acid and 3-sulfophthalic acid in Solvent A Sample solution: 100 mg (± 5 mg) of sample in 10 ml of water
- Test: Appendix Typical HPLC chromatogram of Quinoline Yellow
- Time (min)
Every name it appears under
32 recorded names. A label may use any of them.
E number
- E104
INS number
- 104
CAS registry number
- 8004-92-0
As it appears in regulation
- Quinoline Yellow
Other names
- E 104
- QUINOLINE YELLOW
Systematic names
- Disodium 2-(1,3-dioxoinden-2-yl)quinoline-6,8-disulfonate
The EU re-evaluation programme
Every additive authorised in the EU before 20 January 2009 was put back through EFSA by Commission Regulation (EU) No 257/2010, and this one is named in it. What the Regulation required is below; whether the opinion has since been published is not something this site records.
- Already re-evaluated when the programme began. Listed in Annex I as “Quinoline Yellow”, against the words “Re-evaluation completed on 23 September 2009”, and therefore not re-evaluated again under it.
Hazard reference values
The two committees set different figures. EFSA's acceptable daily intake is 0.5 mg/kg bw/day (2015); JECFA's is 3 mg/kg bw/day (2016). An acceptable daily intake is a reference value set by a committee, not a threshold above which harm begins. The lower figure is not a finding that the other body was wrong: the two work to different terms of reference, on their own timetables, and sometimes from different data. This site states both and chooses between them nowhere. See where the two committees differ.
Evidence and how bodies read it
Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial
- Design
- RCT
- Model
- human
- Dose
- Two mixtures of four synthetic colours plus sodium benzoate, at doses chosen to reflect typical UK intakes, given in a drink; challenge and placebo periods.
- Endpoint
- Global hyperactivity aggregate, combining teacher and parent ratings with a computerised attention test.
- Funding
- UK Food Standards Agency
What it found. Reported a small increase in the composite hyperactivity measure in 3-year-olds and 8/9-year-olds during the additive-challenge periods compared with placebo. The authors described the effects as modest and variable between individuals.
Limitations. The additives were given as mixtures, so the result cannot be attributed to any single colour or to the benzoate. Effect sizes were small relative to the spread between children, adherence differed between age groups, and the trial measured behaviour over short challenge periods rather than long-term outcomes.
How different bodies read the evidence
Each conclusion is paraphrased in the body's own framing, from the published opinion linked beneath it. Where bodies differ, both stand; this site does not adjudicate between them or add a view of its own.
EFSA 2015
In a refined exposure assessment carried out after the European Commission reduced or withdrew maximum permitted levels for Quinoline Yellow (E 104) from 2013, EFSA found that mean and high-level exposure estimates were below the ADI of 0.5 mg/kg bw/day for all population groups in all scenarios.
EFSA 2009
The ANS Panel concluded that the toxicity database, including a rat study not apparently considered by earlier committees, justified redefining the ADI for Quinoline Yellow (E 104), and set it at 0.5 mg/kg bw/day (previously 10 mg/kg bw/day). It noted that refined intake estimates at maximum levels of use were generally well above the new ADI.
EFSA 2008
Judged the trial to provide limited evidence of a small effect on activity and attention in some children, but held that the mixtures tested did not allow any effect to be attributed to an individual additive, and that the findings did not warrant changing the acceptable daily intakes.
Action taken: Left intake limits unchanged
European Union (legislature) 2008
Took the same evidence as grounds for a consumer-information measure rather than a change in permitted levels, requiring foods containing these colours to carry the statement that they 'may have an adverse effect on activity and attention in children'.
Action taken: Imposed a mandatory warning statement while leaving the additives authorised
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is E 104?
- E 104 is Quinoline yellow. Quinoline Yellow is a synthetic greenish-yellow dye used in soft drinks, confectionery and desserts. It is one of the six colours that must carry the activity-and-attention warning in the EU.
- What is Quinoline yellow used for in food?
- It is listed as a colour — the role Codex and the EU assign it. This page shows every function each regulator records, with the citation for each.
- Where is Quinoline yellow permitted?
- Of the seven regulators this site reads, Australia and New Zealand, the EU, Great Britain and the Codex standard permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is Quinoline yellow vegan?
- Yes. Quinoline yellow is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- anz retrieved 2026-10-17
- eu retrieved 2026-10-18
- gb retrieved 2026-10-18
- gsfa retrieved 2026-09-07
- euspecs retrieved 2026-10-18
- jecfaspecs retrieved 2026-09-23
Full licence terms and publisher details on Sources. How this page is built and updated: Method.
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