Rebaudioside M from fermentation produced by Yarrowia lipolytica

E number E960b(i)

Listed as a sweetener by the EU.

Listed as sweeteners (EU)

Rebaudioside M from fermentation produced by Yarrowia lipolytica is a steviol glycoside, rebaudioside M, made by fermentation with the yeast Yarrowia lipolytica. The EU's specification (Regulation 231/2012) numbers it E 960b(i), a form under the E 960b listing in Annex II, Steviol glycosides from fermentation.

Where it is and isn’t permitted

Status of Rebaudioside M from fermentation produced by Yarrowia lipolytica by jurisdiction. Every jurisdiction this site covers is listed, including those where the substance does not appear.
JurisdictionStatusScope and limitsCitation
Australia and New ZealandFood Standards Australia New ZealandNo listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it.
CanadaHealth CanadaNo listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here.
European UnionEuropean Commission / EFSAPermitted, with conditions

34 food categories

highest permitted level 3,300 mg/l

Show every food category
  • 1.4 flavoured fermented milk products including heat-treated products — 100 mg/lAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 100 mg/l. only energy-reduced products or with no added sugar’;.
  • 11.4.1 table-top sweeteners in liquid form — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. quantum satis.
  • 11.4.2 table-top sweeteners in powder form — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. quantum satis.
  • 11.4.3 table-top sweeteners in tablets — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. quantum satis.
  • 12.4 Mustard — 120 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 120 mg/kg.
  • 12.5 Soups and broths — 40 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 40 mg/kg. only energy-reduced soups’.
  • 12.6 Sauces — 175 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 175 mg/kg. Only soy-bean sauce (fermented and non-fermented)’;.
  • 13.2 Foods for special medical purposes as defined by Regulation (EU) No 609/2013 (excluding products from food category 13.1.5.) — 330 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 330 mg/kg.
  • 13.3 Total diet replacement for weight control food as defined by Regulation (EU) No 609/2013 — 270 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 270 mg/kg.
  • 14.1.3 Fruit nectars as defined by Directive 2001/112/EC and vegetable nectars and similar products — 100 mg/lAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 100 mg/l. only energy-reduced or with no added sugar.
  • 14.1.4 Flavoured_drinks — 80 mg/lAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 80 mg/l. only energy-reduced or with no added sugar.
  • 14.1.5.2 other — 20 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 20 mg/kg. only malt-based and chocolate/ cappuccino flavoured drinks, energyreduced or with no added sugars’;.
  • 14.2.1 beer and malt beverages — 70 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 70 mg/kg. only alcohol-free beer or with an alcohol content not exceeding 1,2 % vol.; ‘Bière de table/Tafelbier/Table beer’ (original wort content less than 6 %) except for ‘Obergäriges Einfachbier’; beers with a minimum acidity of 30 milli-equivalents expressed as NaOH; Brown beers of the ‘oud bruin’ type’;.
  • 14.2.8 Other alcoholic beverages including mixtures of alcoholic beverages with non-alcoholic beverages and other alcoholic beverages based on distilled alcohol with alcoholic strength by volume less than 15 % — 150 mg/lAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 150 mg/l.
  • 15.1 Potato- cereal- flour- or starch-based snacks — 20 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 20 mg/kg.
  • 15.2 Processed nuts — 20 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 20 mg/kg.
  • 16 Desserts excluding products covered in category 1 3 and 4 — 100 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 100 mg/kg. only energy-reduced or with no added sugar’;.
  • 17.1 food supplements supplied in a solid form excluding food supplements for infants and young children’, — 1,800 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 1800 mg/kg. only food supplements in chewable form.
  • 17.2 food supplements supplied in a liquid form excluding food supplements for Infants and young children — 1,800 mg/lAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 1800 mg/l. only food supplements in syrup form’.
  • 18.2 Meal replacement for weight control as referred to in Commission Regulation (EU) No 432/2012 — 270 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 270 mg/kg.
  • 3 edible ices — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only energy-reduced products or with no added sugar’.
  • 4.2.2 fruit and vegetables in vinegar oil or brine — 100 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 100 mg/kg. only sweet-sour preserves of fruit and vegetables’;.
  • 4.2.4.1 fruit and vegetable preparations excluding compote — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only energy-reduced’;.
  • 4.2.5.1 Extra jam and extra jelly as defined by Directive 2001/113/EC — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only energy-reduced jams jellies and marmalades’;.
  • 4.2.5.2 Jam, jellies and marmalades and sweetened chestnut puree as defined by Directive 2001/113/EC — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only energy-reduced jams jellies and marmalades’;.
  • 4.2.5.3 other similar fruit or vegetable spreads — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only energy-reduced fruit or vegetable spreads and dried-fruit-based sandwich spreads, energy-reduced or with no added sugar’;.
  • 5.1 Cocoa and chocolate products as covered by Directive 2000/36/EC — 270 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 270 mg/kg. only energy-reduced or with no added sugars’;.
  • 5.2 Other confectionery including breath freshening micro-sweets — 270 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. ML = 270 mg/kg. only cocoa or dried fruit based, energy reduced or with no added sugar.
  • 5.2 Other confectionery including breath freshening micro-sweets — 670 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 670 mg/kg. only strongly flavoured freshening throat pastilles, energy-reduced or with no added sugars’.
  • 5.3 chewing gum — 3,300 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 3300 mg/kg. only with no added sugar’;.
  • 5.4 Decorations, coatings and fillings, except fruit-based fillings covered by category 4.2.4 — 270 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 270 mg/kg. only cocoa or dried fruit based, energy reduced or with no added sugar’;.
  • 6.3 breakfast cereals — 330 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 330 mg/kg. only breakfast cereals with a fibre content of more than 15 %, and containing at least 20 % bran, energy reduced or with no added sugar’.
  • 7.2 Fine bakery wares — 330 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 330 mg/kg. only essoblaten – wafer paper.
  • 9.2 Processed fish and fishery products including molluscs and crustaceans — 200 mg/kgAuthorised under E 960b, Steviol glycosides from fermentation’. Permitted as a member of Steviol glycosides. ML = 200 mg/kg. only sweet-sour preserves and semi preserves of fish and marinades of fish, crustaceans and molluscs’;.
Regulation (EC) No 1333/2008, Annex IIand 33 further provisionsretrieved 2026-10-18
Great BritainFood Standards Agency / Food Standards ScotlandPermitted, with conditions

32 food categories

highest permitted level 3,330 mg/kg or mg/l

Show every food category
  • 1.4 Flavoured fermented milk products including heat-treated products — 100 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 100. only energy-reduced products or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 11.4.1 Table-top sweeteners in liquid form — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation. quantum satis. Note (60): Expressed as steviol equivalents.
  • 11.4.2 Table-top sweeteners in powder form — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation. quantum satis. Note (60): Expressed as steviol equivalents.
  • 11.4.3 Table-top sweeteners in tablets — quantum satisAuthorised under E 960b, Steviol glycosides from fermentation. quantum satis. Note (60): Expressed as steviol equivalents.
  • 12.4 Mustard — 120 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 120. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 12.5 Soups and broths — 40 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 40. only energy-reduced soups. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 12.6 Sauces — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 120: except soy-bean sauce (fermented and non-fermented); (2) ML 175: only soy-bean sauce (fermented and non-fermented). Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 13.2 Dietary foods for special medical purposes defined in Directive 1999/21/EC (excluding products from food category 13.1.5) — 330 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 330. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 13.3 Dietary foods for weight control diets intended to replace total daily food intake or an individual meal (the whole or part of the total daily diet) — 270 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 270. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 14.1.3 Fruit nectars as defined by Directive 2001/112/EC and vegetable nectars and similar products — 100 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 100. only energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 14.1.4 Flavoured drinks — 80 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 80. only energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 14.1.5.2 Other — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 30: only coffee, tea and herbal infusion beverages, energy-reduced or with no added sugar; (2) ML 30: only flavoured instant coffee and instant cappuccino products, energy-reduced or with no added sugar; (3) ML 20: only malt-based and chocolate/cappuccino flavoured drinks, energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents. Note (93): Maximum level applies to the ready-to-drink products (e.g. canned) and their mixes and concentrates after preparation and ready for consumption.
  • 14.2.1 Beer and malt beverages — 70 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 70. only alcohol-free beer or with an alcohol content not exceeding 1.2% volume.; ‘Bière de table/Tafelbier/Table beer’ (original wort content less than 6%) except for ‘Obergäriges Einfachbier’; beers with a minimum acidity of 30 milli-equivalents expressed as NaOH; Brown beers of the ‘oud bruin’ type. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 14.2.8 Other alcoholic drinks including mixtures of alcoholic drinks with non-alcoholic drinks and spirits with less than 15 % of alcohol — 150 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 150. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 15.1 Potato-, cereal-, flour- or starch-based snacks — 20 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 20. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 15.2 Processed nuts — 20 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 20. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 16 Desserts excluding products covered in categories 1, 3 and 4 — 100 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 100. only energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 17.1 Food supplements supplied in a solid form, excluding food supplements for infants and young children — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 670: no further restriction; (2) ML 1800: only food supplements in chewable form. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 17.2 Food supplements supplied in a liquid form, excluding food supplements for infants and young children — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 200: no further restriction; (2) ML 1800: only food supplements in syrup form. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 3 Edible ices — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only energy-reduced products or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 4.2.2 Fruit and vegetables in vinegar, oil, or brine — 100 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 100. only sweet-sour preserves of fruit and vegetables. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 4.2.4.1 Fruit and vegetable preparations excluding compote — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only energy-reduced. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 4.2.5.1 Extra jam and extra jelly as defined by Directive 2001/113/EC — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only energy-reduced jams, jellies and marmalades. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 4.2.5.2 Jam, jellies and marmalades and sweetened chestnut puree as defined by Directive 2001/113/EC — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only energy-reduced jams, jellies and marmalades. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 4.2.5.3 Other similar fruit or vegetable spreads — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only energy-reduced fruit or vegetable spreads and dried-fruit-based sandwich spreads, energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 5.1 Cocoa and chocolate products as covered by Directive 2000/36/EC — 270 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 270. only energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 5.2 Other confectionery including breath refreshening microsweets — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 270: only cocoa or dried fruit based; energy-reduced or with no added sugar; (2) ML 330: sandwich spreads made with a base of cocoa, milk, dried fruit or fat; energy-reduced or with no added sugar; (3) ML 350: only confectionery with no added sugar; only energy-reduced hard confectionery such as candies and lollies; only energy-reduced soft confectionery such as chewy candies, fruit gums and foam sugar products/marshmallows; only energy-reduced liquorice; only energy-reduced nougat; only energy-reduced marzipan; (4) ML 2000: only breath-freshening microsweets, energy-reduced or with no added sugar; (5) ML 670: only strongly flavoured freshening throat pastilles, energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 5.3 Chewing gum — 3,330 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 3330. only with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 5.4 Decorations, coatings and fillings, except fruit based fillings covered by category 4.2.4 — several limitsAuthorised under E 960b, Steviol glycosides from fermentation. (1) ML 330: only confectionery with no added sugar; (2) ML 270: only cocoa or dried fruit based; energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 6.3 Breakfast cereals — 330 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 330. only breakfast cereals with a fibre content of more than 15%, and containing at least 20% bran, energy-reduced or with no added sugar. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 7.2 Fine bakery wares — 330 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 330. only essoblaten – wafer paper. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
  • 9.2 Processed fish and fishery products including mollusks and crustaceans — 200 mg/kg or mg/lAuthorised under E 960b, Steviol glycosides from fermentation. ML = 200. only sweet-sour preserves and semi preserves of fish and marinades of fish, crustaceans and molluscs. Note (1): The additives may be added individually or in combination. Note (60): Expressed as steviol equivalents.
FSA/FSS Register of Food Additive Authorisations, E 960a–c conditions of use (19 February 2026), under E 960band 31 further provisionsretrieved 2026-10-18
JapanConsumer Affairs AgencyNo listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance.
South KoreaMinistry of Food and Drug SafetyNo listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance.
United StatesFood and Drug AdministrationNot listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited.
Codex AlimentariusCodex Alimentarius Commission / JECFANot listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited.

How much is permitted

A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.

European Union20 mg/kg – 3,300 mg/kg31 numeric limit, 3 quantum satis
Great Britain20 mg/kg – 3,330 mg/kg23 numeric limit, 6 several limits, 3 quantum satis

What a label must say

Words a food containing Rebaudioside M from fermentation produced by Yarrowia lipolytica is obliged to carry, quoted from the instrument that imposes them. A required statement is a fact about the regulation, not a finding about the substance — every rule below applies where the additive is authorised.

Every rule collected at what a label must say.

What it is made from

The sources cited here do not say how it is made, so this page claims no route.

How the EU defines it

The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.

E 960b(i) Rebaudioside m from fermentation produced by yarrowia lipolytica

Rebaudioside M from fermentation produced by Yarrowia lipolytica consist of a mixture of steviol glycosides composed of rebaudioside M as the main componant, with some rebaudioside D, and smaller amounts of rebaudioside A and rebaudioside B. The manufacturing process comprises two main phases. The first phase involves fermentation of a simple sugar source by a non-toxigenic non-pathogenic strain of Yarrowia lipolytica that has been genetically modified with heterologous genes to overexpress genes which are involved in the synthesis of steviol glycosides to result in the strain VRM (CBS 147477). Removal of biomass by solid-liquid separation and heat treatment is followed by concentration of the steviol glycosides. The second phase involves purification by employing ion exchange chromatography, followed by cristallisation of the steviol glycosides from ethanol, resulting in a final product containing not less than 95 % of rebaudiosides M, D, A and B. Viable cells and DNA of Yarrowia lipolytica VRM shall not be detected in the food additive.

Assay, identification and purity criteria

Description and assay

Chemical name
Rebaudioside A: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D- glucopyranosyl ester Rebaudioside B: 13-[(2-O-β–D-glucopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid Rebaudioside D: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester Rebaudioside M: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester
Molecular formula
Trivial name Formula Conversion factor Rebaudioside A C₄₄ H₇₀ O₂₃ 0,33
Rebaudioside B
C₃₈ H₆₀ O₁₈ 0,40
Rebaudioside D
C₅₀H₈₀O₂₈ 0,29
Rebaudioside M
C₅₆H₉₀O₃₃ 0,25
Molecular weight and CAS No
Trivial name CAS Number Molecular weight (g/mol)
Rebaudioside A
58543-16-1 967,01
Rebaudioside B
58543-17-2 804,88
Rebaudioside D
63279-13-0 1 129,15
Rebaudioside M
1220616-44-3 1 291,30
Assay
Not less than 95 % of rebaudioside M, rebaudioside D, rebaudioside A and rebaudioside B, on the dried basis.
Particle size
Not more than 0,5 % of particles of less than 300 nm (by STEM method)
Description
White to light yellow powder, approximately between 200 and 350 times sweeter than sucrose (at 5 % sucrose equivalency). Identification
Solubility
Slightly soluble in water
pH
Between 4,5 and 7,0 (1 in 100 solution) Purity
Total ash
Not more than 1 %
Loss on drying
Not more than 6 % (105 °C, 2h)
Residual solvent
Not more than 5 000 mg/kg ethanol
Kaurenoic acid
Not more than 0,3 mg/kg (measured by liquid chromatography-mass spectrometry detection)
Arsenic
Not more than 0,01 mg/kg
Lead
Not more than 0,01 mg/kg
Cadmium
Not more than 0,01 mg/kg
Mercury
Not more than 0,05 mg/kg Microbiological criteria
Total (aerobic) plate count
Not more than 1 000 CFU/g
Yeast and moulds
Not more than 100 CFU/g
Escherichia coli
Negative in 1 g
Salmonella spp.
Negative in 25 g
Residual protein
Not more than 20 mg/kg

Commission Regulation (EU) No 231/2012, Annex, E 960b(i)

Every name it appears under

3 recorded names. A label may use any of them.

E number

  • E960b

As it appears in regulation

  • Steviol glycosides from fermentation’

Other names

  • Rebaudioside M from fermentation produced by Yarrowia lipolytica

Hazard reference values

No reference value for this substance in the sources we hold.

Evidence and how bodies read it

Studies, and the differing conclusions regulators drew from them, are curated rather than machine-read, and none has been written for this substance yet.

Common questions

Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.

What is E 960b(i)?
E 960b(i) is Rebaudioside M from fermentation produced by Yarrowia lipolytica. Rebaudioside M from fermentation produced by Yarrowia lipolytica is a steviol glycoside, rebaudioside M, made by fermentation with the yeast Yarrowia lipolytica. The EU's specification (Regulation 231/2012) numbers it E 960b(i), a form under the E 960b listing in Annex II, Steviol glycosides from fermentation.
What is Rebaudioside M from fermentation produced by Yarrowia lipolytica used for in food?
It is listed as a sweetener — the role the EU assigns it. This page shows every function each regulator records, with the citation for each.
Where is Rebaudioside M from fermentation produced by Yarrowia lipolytica permitted?
Of the seven regulators this site reads, the EU and Great Britain permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.

Sources for this page

  • eu retrieved 2026-10-18
  • gb retrieved 2026-10-18
  • euspecs retrieved 2026-10-18

Full licence terms and publisher details on Sources. How this page is built and updated: Method.

Machine-readable: a JSON record of this additive is given on request —how to ask for access.

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