Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed)
Enzymatically hydrolysed carboxy methyl cellulose · SODIUM CARBOXYMETHYL CELLULOSE, ENZYMATICALLY HYDROLYSED (CELLULOSE GUM, ENZYMATICALLY HYDROLYZED) · E469 · 469 · Enzymatically hydrolysed carboxy methyl cellulose · Carbossimetilcellulosa idrolizzata enzimaticamente, gomma di cellulosa idrolizzata enzimaticamente · CARBOXIMETILCELULOSA HIDROLIZADA ENZIMÁTICAMENTE, GOMA DE CELULOSA HIDROLIZADA ENZIMÁTICAMENTE · Carboximetilcelulosa sódica hidrolizada enzimáticamente, goma de celulosa hidrolizada enzimáticamente · Carboximetilcelulose hidrolisada enzimaticamente, goma de celulose hidrolisada enzimaticamente · Carboximetilceluloză hidrolizată enzimatic, gumă de celuloză hidrolizată enzimatic · Carboxyméthylcellulose hydrolysée de manière enzymatique, gomme de cellulose hydrolysée de manière enzymatique · encimsko hidrolizirana karboksimetil celuloza · ENCIMSKO HIDROLIZIRANA KARBOKSIMETIL CELULOZA, ENCIMSKO HIDROLIZIRANI CELULOZNI GUMI · ENSÜMAATILISELT HÜDROLÜÜSITUD KARBOKSÜMETÜÜLTSELLULOOS · Ensüümide abil hüdrolüüsitud karboksümetüültselluloos · Entsymaattisesti hydrolysoitu karboksimetyyliselluloosa, entsymaattisesti hydrolysoitu selluloosakumi · Enzimatski hidrolizirana karboksi metil celuloza, enzimatski hidrolizirana celulozna guma · Enzimesen hidrolizált karboxi-metil-cellulóz, enzimesen hidrolizált cellulózgumi · ENZIMSKI HIDROLIZIRANA KARBOKSIMETILCELULOZA, ENZIMSKI HIDROLIZIRANA CELULOZNA GUMA · Enzymatically hydrolysed carboxy methyl cellulose, Enzymatically hydrolysed cellulose gum · ENZYMATICALLY HYDROLYSED CARBOXYMETHYLCELLULOSE, ENZYMATICALLY HYDROLISED CELLULOSE GUM · Enzymaticky hydrolyzovaná karboxymetylcelulóza, enzymaticky hydrolyzovaná celulózová guma · Enzymatisch gehydrolyseerde carboxymethylcellulose, enzymatisch gehydrolyseerde cellulosegom · Enzymatisch hydrolysierte Carboxymethylcellulose · ENZYMATISCH HYDROLYSIERTE CARBOXYMETHYLCELLULOSE, ENZYMATISCH HYDROLYSIERTER CELLULOSEGUMMI · ENZYMATISK HYDROLISERET CARBOXYMETHYLCELLULOSE (ENZYMATISK HYDROLYSERET CELLULOSEGUMMI) · Enzymatisk hydrolyseret carboxymethylcellulose (enzymatisk hydrolyseret cellulosegummi) · Enzymatiskt hydrolyserad karboximetylcellulosa, enzymatiskt hydrolyserat cellulosagummi · Enzymatycznie zhydrolizowana karboksymetyloceluloza, enzymatycznie zhydrolizowana guma celulozowa · Enzymově hydrolyzovaná karboxymethylcelulosa, enzymově hydrolyzovaná celulosová guma · Fermentais hidrolizuota karboksimetilceliuliozė, fermentais hidrolizuota celiuliozės derva · FERMENTATĪVI HIDROLIZĒTA KARBOKSIMETILCELULOZE, FERMENTATĪVI HIDROLIZĒTI CELULOZES SVEĶI · Fermentatīvi hidrolizēta karboksimetilceluloze, fermentatīvi hidrolizētas celulozes sveķi · KARBOSSIMETILĊELLULOŻA IDROLIZZATA BL-ENŻIMI, GOMMA TAĊ-ĊELLULOŻA IDROLIZZATA BL-ENŻIMI · Karbossimetilċelluloża idrolizzata b'mod enżimatiku, gomma taċ-ċelluloża idrolizzata b'mod enżimatiku · Ενζυμικά υδρολυμένη καρβοξυμεθυλοκυτταρίνη, ενζυμικά υδρολυμένο κόμμι κυτταρίνης · Ензимно хидролизирана карбоксиметил целулоза, ензимно хидролизирана целулозна гумаE number E469INS 469
Listed as a thickener and a stabiliser by Codex.
Listed as thickeners (Codex) · stabilisers (Codex)
- Codex Alimentarius: Thickener — CXS 192-1995 Table One, INS 469
- Codex Alimentarius: Stabilizer — CXS 192-1995 Table One, INS 469
Enzymatically hydrolysed cellulose gum is a shorter-chain version of the familiar thickener, giving lower viscosity at the same concentration — useful where the stabilising effect is wanted without the thickness.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | No listed food uses foundThis site has not matched this substance to any food use on this jurisdiction’s list of authorised food additives. A food additive may be used only in foods that list names. Flavourings, enzymes, processing aids and added nutrients are regulated separately and do not appear on it. | ||
| Canada | No listing matchedHealth Canada’s lists name additives without E or INS numbers, so this site matches them by exact name. A substance listed there under a name this site does not recognise has no row here. | ||
| European Union | Permitted, with conditions | 68 food categories Show every food category
| Regulation (EC) No 1333/2008, Annex IIand 67 further provisionsretrieved 2026-10-18 |
| Great Britain | Permitted, with conditions | 66 food categories Show every food category
| Assimilated Regulation (EC) No 1333/2008, Annex II (as at 28 June 2024)and 65 further provisionsretrieved 2026-10-18 |
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | No listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance. | ||
| United States | Not listedDoes not appear on this jurisdiction’s permitted list. That is not the same as being prohibited. | ||
| Codex Alimentarius | Permitted, with conditions | 2 food categories no numerical limit (good manufacturing practice) Show every food category and its notes
| CXS 192-1995 Table Three, INS 469and 2 further provisionsretrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| European Union | — | 46 several limits, 22 no quantity stated |
|---|---|---|
| Great Britain | — | 66 quantum satis |
| Codex Alimentarius | — | 3 GMP |
No regulator sets a figure for Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed). That is not an absence of control: where the listing says quantum satis or good manufacturing practice, the quantity is limited to what the effect requires.
What it is made from
Made by treating cellulose gum with cellulase enzymes to cut the chains to a controlled length.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
The two specifications, compared
The European Union sets identity and purity by regulation; JECFA publishes its own through FAO, advisory until a country adopts it. Both documents are below in full. These are the parameters each puts a number on.
| Parameter | European Union | JECFA |
|---|---|---|
| Assay | no less than 99.5 % | no less than 99.5 % |
| Loss on drying | no more than 12 % | no more than 12 % |
| Lead | no more than 0.1 mg/kg | no more than 3 mg/kg |
| Arsenic | no more than 0.1 mg/kg | — |
| Mercury | no more than 0.1 mg/kg | — |
| Cadmium | no more than 0.1 mg/kg | — |
One parameter differs between the two documents. A difference is not a disagreement about safety: the two are revised on their own cycles, and an assay or a drying loss can be measured against a different preparation of the substance, which is why only the contaminant ceilings are marked. Within the EU the EU's figure is the one that binds.
How the EU defines it
The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.
E 469 Enzymatically hydrolysed carboxymethylcellulose, enzymatically hydrolised cellulose gum
Enzymatically hydrolysed carboxymethylcellulose is obtained from carboxymethylcellulose by enzymatic digestion with a cellulase produced by Trichoderma longibrachiatum (formerly T. reesei)
Synonyms: Sodium carboxymethyl cellulose, enzymatically hydrolysed
Assay, identification and purity criteria
Description and assay
- Chemical name
- Carboxymethyl cellulose, sodium, partially enzymatically hydrolysed
- Chemical formula
- Sodium salts of polymers containing substituted anhydroglucose units with the general formula: [C₆H₇O₂(OH)ₓ(OCH₂COONa)y]ₙ where n is the degree of polymerisation x = 1,50 to 2,80 y = 0,2 to 1,50 x + y = 3,0 (y = degree of substitution)
- Molecular weight
- 178,14 where y = 0,20 282,18 where y = 1,50 Macromolecules: Not less than 800 (n about 4)
- Assay
- Not less than 99,5 %, including mono- and disaccharides, on the dried basis
- Description
- White or slightly yellowish or greyish, odourless, slightly hygroscopic granular or fibrous powder
Identification
- Solubility
- Soluble in water, insoluble in ethanol
- Foam test
- Vigorously shake a 0,1 % dispersion of the sample. No layer of foam appears. This test distinguishes sodium carboxymethyl cellulose, whether hydrolysed or not, from other cellulose ethers and from alginates and natural gums
- Precipitate formation
- To 5 ml of a 0,5 % dispersion of the sample, add 5 ml of a 5 % solution of copper or aluminium sulphate. A precipitate appears. This test distinguishes sodium carboxymethyl cellulose, whether hydrolysed or not, from other cellulose ethers and from gelatine, carob bean gum and tragacanth gum
- Colour reaction
- Add 0,5 g of the powdered sample to 50 ml of water, while stirring to produce a uniform dispersion. Continue the stirring until a clear dispersion is produced. Dilute 1 ml of the dispersion with 1 ml of water in a small test tube. Add 5 drops of 1-naphthol TS. Incline the tube, and carefully introduce down the side of the tube 2 ml of sulphuric acid so that it forms a lower layer. A red-purple colour develops at the interface
- Viscosity (60 % solids)
- Not less than 2 500 kgm– 1s– 1 at 25 °C corresponding to an average molecule weight of 5 000 Da
- pH
- Not less than 6,0 and not more than 8,5 (1 % colloidal dispersion)
Purity criteria
- Loss on drying
- Not more than 12 % (105 °C to constant weight)
- Degree of substitution
- Not less than 0,2 and not more than 1,5 carboxymethyl groups per anhydroglucose unit on the dried basis
- Sodium chloride and sodium glycolate
- Not more than 0,5 % singly or in combination
- Residual enzyme activity
- Passes test. No change in viscosity of test dispersion occurs, which indicates hydrolysis of the sodium carboxymethyl cellulose
- Arsenic
- Not more than 0,1 mg/kg
- Lead
- Not more than 0,1 mg/kg
- Mercury
- Not more than 0,1 mg/kg
- Cadmium
- Not more than 0,1 mg/kg
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
INS 469 Sodium Carboxymethyl Cellulose, enzymatically hydrolyzed
The product is the sodium salt of a carboxymethyl ether of cellulose, which has been partially hydrolyzed by enzymatic treatment with food-grade Trichoderma reesei cellulase. The total content of mono- and disaccharides does not exceed about 7.5%.
Synonyms: Enzymatically hydrolyzed carboxymethyl cellulose; CMC-ENZ; INS No.
Assay, identification and purity criteria
Description and assay
- Specification history
- SODIUM CARBOXYMETHYL CELLULOSE, ENZYMATICALLY HYDROLYZED Prepared at the 51st JECFA (1998), published in FNP 52 Add 6 (1998) superseding tentative specifications prepared at the 49th JECFA (1997), published in FNP 52 Add 5 (1997). This substance was included at the 51st JECFA (1998) in the group ADI "not specified" for modified celluloses, established at the 35th JECFA in 1989.
- Chemical names
- Carboxymethyl cellulose, sodium, partially enzymatically hydrolyzed
- Chemical formula
- Sodium salts of polymers containing substituted anhydroglucose units with the general formula: [C 6 H 7 O 2 (OH) x (OCH 2 COONa) y ] n where n is the degree of polymerization x = 1.50 to 2.80 y = 0.2 to 1.50 x + y = 3.0 (y = degree of substitution)
- Structural formula
- where R = H, CH 2 COONa or CH 2 COOH
- Formula weight
- 178.14, where y = 0.20 282.18, where y = 1.50 Macromolecules: Not less than 800 (n about 4)
- Assay
- Not less than 99.5%, including mono- and disaccharides, on the dried basis
- DESCRIPTION
- White or slightly yellowish or greyish, odourless, slightly hygroscopic granular or fibrous powder
- FUNCTIONAL USES
- Carrier, glazing agent, stabilizer, thickener
- METHOD OF ASSAY
- Calculate the percentage of enzyme treated sodium carboxymethyl cellulose by subtracting from 100 the sum of the percentages of sodium chloride and sodium glycolate, determined separately by the procedures above.
Identification
- Solubility (Vol. 4)
- Soluble in water; insoluble in ethanol
- Foam test
- Vigorously shake a 0.1% solution of the sample. No layer of foam appears. This test distinguishes sodium carboxymethyl cellulose, whether hydrolysed or not, from other cellulose ethers and from alginates and natural gums.
- Precipitate formation
- To 5 ml of a 0.5% solution of the sample add 5 ml of a 5% solution of copper or aluminium sulfate. A precipitate appears. This test distinguishes sodium carboxymethyl cellulose, whether hydrolysed or not, from other cellulose ethers, and from gelatine, carob bean gum and tragacanth gum.
- Colour reaction
- Add 0.5 g of the powdered sample to 50 ml of water, while stirring to produce a uniform dispersion. Continue the stirring until a clear solution is produced. Dilute 1 ml of the solution with 1 ml of water in a small test tube. Add 5 drops of 1-naphthol TS. Incline the tube, and carefully introduce down the side of the tube 2 ml of sulphuric acid so that it forms a lower layer. A red-purple colour develops at the interface.
- Viscosity (60% solids)
- Not less than 2500 mPas corresponding to an average molecular weight of 5000 D. This test also distinguishes enzymatically hydrolyzed CMC from non-hydrolyzed CMC since it is not possible to make a 60% solution of ordinary CMC. See description under TESTS
- Test: Viscosity (60% solids)
- To 40 ml of water, add 60 g of the sample, stirring continuously. To ensure the formation of a clear solution without air bubbles, leave the solution to stand in a refrigerator (+4 o ) for several hours. Using a Bohlin viscometer or an equivalent instrument, measure the viscosity of the solution at 25 o using a shear rate of 147sec -1 .
Purity criteria
- Loss on drying (Vol. 4)
- Not more than 12% (105 o to constant weight)
- pH (Vol. 4)
- Not less than 6.0 and not more than 8.5 (1 in 100 solution)
- Sodium chloride and sodium glycolate
- Not more than 0.5%, singly or in combination See descriptions under TESTS
- Degree of substitution
- Not less than 0.2 and not more than 1.50 carboxymethyl groups (CH 2 COOH) per anhydroglucose unit on the dried basis See description under TESTS
- Residual enzyme activity
- Passes test See description under TESTS
- Lead (Vol. 4)
- Not more than 3 mg/kg Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, “Instrumental Methods.”
- Test: Sodium chloride
- Heat 5 g of the sample, weighed to the nearest 0.1 mg, in a platinum or porcelain crucible, first with a small flame so that the sample does not ignite and then, when the charring is complete, heat further in an electric oven for 15 min at about 500 o . After cooling, pulverize the ashes thus obtained and extract several times with warm water. Filter the extracts into a 500-ml volumetric flask, acidify with nitric acid and dilute to the mark with water. Determine the NaCl content of 100 ml of this extract by the method of Volhard, using 0.2 N silver nitrate and 0.02 N ammonium thiocyanate. Each ml of 0.02 N silver nitrate is equivalent to 1.169 mg of NaCl. Calculate the sodium chloride content by the formula: % NaCl = [(a x 0.001169 x 5)/b] x 100 where a = 0.02 N silver nitrate consumed (ml) b = dry weight of the sample (g)
- Test: Sodium glycolate
- Proceed as directed under Chromatography (High Performance Liquid Chromatography) using the following conditions and using pure glycolic acid as the reference substance: Column: Two cation exchange columns in the H + -form in series, e.g. two Fast Fruit Juice columns, 15 cm x 7.8 mm, Waters, or equivalent Elution: Isocratic Mobile phase: Aqueous 0.05% phosphoric acid Flow: 0.5 ml/min Detector type: UV or diode array, 205 nm Sample size: 50 μl of a solution of 200.0 mg of the sample in 20 ml of eluent
- Test: Degree of substitution
- Weigh accurately about 200 mg of the sample, previously dried at 105 o to constant weight, and transfer it into a 250-ml glass-stoppered Erlenmeyer flask. Add 75 ml of glacial acetic acid, and connect the flask with a water-cooled condenser, and reflux gently on a hot plate for 2 h. Cool, transfer the solution to a 250-ml beaker with the aid of 50 ml of glacial acetic acid, and titrate with 0.1 N perchloric acid in dioxane while stirring with a magnetic stirrer. Determine the endpoint potentiometrically with a pH meter equipped with a standard glass electrode and a calomel electrode modified as follows: Discard the aqueous potassium chloride solution, rinse and fill with the supernatant liquid obtained by shaking thoroughly 2 g each of potassium chloride and silver chloride (or silver oxide) with 100 ml of methanol, then add a few crystals of potassium chloride and silver chloride (or silver oxide) to the electrode. Record the ml of 0.1 N perchloric acid versus mV (0 to 700 mV range), and continue the titration to a few ml beyond the endpoint. Plot the titration curve, and read the volume (A), in ml, of 0.1 N perchloric acid at the inflection point. Calculate the degree of substitution (DS) by the formula (16.2 A/G)/[1.000 - (8.0 A/G)], where A = the volume of 0.1 N perchloric acid required (ml) G = weight of the sample taken (mg) 16.2 = one-tenth of the formula weight of one anhydroglucose unit 8.0 = one-tenth of the formula weight of one sodium carboxymethyl group
- Test: Residual enzyme activity
- Prepare a 5% solution of sodium carboxymethyl cellulose having a viscosity of 25-50 mPas as a 2% solution. To 20 g of this 5%-solution, add 2 g of a 20% aqueous solution of the sample. Using a Bohlin viscometer or an equivalent instrument, follow the viscosity of the mixture for 10 minutes at 25 o , using a shear rate of 147sec -1 . No change in viscosity (indicating hydrolysis of the sodium carboxymethyl cellulose), occurs. As a control, measure the viscosity of 2 g of water and 20 g of the same sodium carboxymethyl cellulose solution.
Authorised in other additives, enzymes, flavourings and nutrients
These authorisations are not for use in food. They let this substance be used inside another additive, a food enzyme, a flavouring or a nutrient preparation — often as a carrier — and it reaches food only as part of that product. Annex III to Regulation (EC) No 1333/2008.
| Part | May be used in | Maximum level | Citation |
|---|---|---|---|
| Annex III, Part 1: Carriers in food additives | All food additives | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 1 |
| Annex III, Part 2: Food additives other than carriers in food additives | All food additive preparationsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" Note (1): Except enzymes authorised as food additives. | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 2, via Table 1 of Part 6 |
| Annex III, Part 3: Food additives including carriers in food enzymes | Food enzymesNote (1): Including enzymes authorised as food additives. | In enzyme preparation: quantum satis In final food except beverages: quantum satis In beverages: quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 3 |
| Annex III, Part 4: Food additives including carriers in food flavourings | All flavouringsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 4, via Table 1 of Part 6 |
| Annex III, Part 5, Section A: Food additives added in nutrients except in nutrients intended to be used in foodstuffs listed in Part E, point 01.10, of Annex II and foodstuffs for infants and young children listed in Part E, point 13.1, of Annex II | All nutrientsCan be used as a carrier: Yes | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 5, Section A |
Every name it appears under
37 recorded names. A label may use any of them.
E number
- E469
INS number
- 469
As it appears in regulation
- Enzymatically hydrolysed carboxy methyl cellulose
Other names
- Enzymatically hydrolysed carboxy methyl cellulose
- SODIUM CARBOXYMETHYL CELLULOSE, ENZYMATICALLY HYDROLYSED (CELLULOSE GUM, ENZYMATICALLY HYDROLYZED)
Hazard reference values
| Type | Value | Population | Assessed by | Opinion |
|---|---|---|---|---|
| ADI | not specified | — | JECFA | 1998 |
Evidence and how bodies read it
What assessing bodies concluded
Each conclusion is paraphrased in the body's own framing, from the published opinion linked beneath it. Where bodies differ, both stand; this site does not adjudicate between them or add a view of its own.
EFSA 2018
The ANS Panel concluded, using read-across between the celluloses, that there was no genotoxic or carcinogenic concern for unmodified and modified celluloses (E 460–E 469), that no numerical ADI is needed, and that there would be no safety concern at the reported uses and use levels.
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is E 469?
- E 469 is Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed). Enzymatically hydrolysed cellulose gum is a shorter-chain version of the familiar thickener, giving lower viscosity at the same concentration — useful where the stabilising effect is wanted without the thickness.
- What is Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed) used for in food?
- It is listed as a thickener and a stabiliser — the role Codex assigns it. This page shows every function each regulator records, with the citation for each.
- Where is Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed) permitted?
- Of the seven regulators this site reads, the EU, Great Britain and the Codex standard permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed) vegan?
- Yes. Sodium carboxymethyl cellulose, enzymatically hydrolysed (Cellulose gum, enzymatically hydrolyzed) is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- eu retrieved 2026-10-18
- gb retrieved 2026-10-18
- gsfa retrieved 2026-09-07
- euspecs retrieved 2026-10-18
- jecfaspecs retrieved 2026-09-23
Full licence terms and publisher details on Sources. How this page is built and updated: Method.
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