Agar
9002-18-0 · AGAR · E 406 · Gelose · Kanten, Bengal, Ceylon, Chinese or Japanese isinglass · Layor Carang · E406 · 406 · Agar · Agar-agar · Agar · Agar-Agar · Agaragar · Agaras · Agars · Άγαρ · АгарE number E406INS 406CAS 9002-18-0
Listed as an emulsifier, a thickener and a stabiliser by Canada, Codex and the US.
Listed as emulsifiers (CA, US, Codex) · thickeners (CA, US, Codex) · stabilisers (CA, US, Codex) · gelling agents (CA, Codex) · humectants (US, Codex) · glazing and surface-finishing agents (US, Codex) · flavour enhancers (US) · bulking agents (Codex) · flavouring agents and adjuvants (US) · carriers (Codex) · texturisers (US) · formulation aids (US) · processing aids (US)
- Canada: Emulsifying, gelling, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Emulsifier or emulsifier salt — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- Codex Alimentarius: Emulsifier — CXS 192-1995 Table One, INS 406
- Canada: Emulsifying, gelling, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Stabilizer or thickener — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- United States: Stabilizers and thickeners — 21 CFR 184.1115, citing 21 CFR 170.3(o)(28)
- Codex Alimentarius: Thickener — CXS 192-1995 Table One, INS 406
- Canada: Emulsifying, gelling, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Stabilizing agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Stabilizer or thickener — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- United States: Stabilizers and thickeners — 21 CFR 184.1115, citing 21 CFR 170.3(o)(28)
- Codex Alimentarius: Stabilizer — CXS 192-1995 Table One, INS 406
- Canada: Emulsifying, gelling, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Gelling agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Codex Alimentarius: Gelling agent — CXS 192-1995 Table One, INS 406
- United States: Humectants — 21 CFR 184.1115, citing 21 CFR 170.3(o)(16)
- Codex Alimentarius: Humectant — CXS 192-1995 Table One, INS 406
- United States: Surface-finishing agent — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- United States: Surface-finishing agents — 21 CFR 184.1115, citing 21 CFR 170.3(o)(30)
- Codex Alimentarius: Glazing agent — CXS 192-1995 Table One, INS 406
- United States: Flavor enhancer — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- Codex Alimentarius: Bulking agent — CXS 192-1995 Table One, INS 406
- United States: Flavoring agents and adjuvants — 21 CFR 184.1115, citing 21 CFR 170.3(o)(12)
- Codex Alimentarius: Carrier — CXS 192-1995 Table One, INS 406
- United States: Texturizer — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
- United States: Formulation aids — 21 CFR 184.1115, citing 21 CFR 170.3(o)(14)
- United States: Processing aid — FDA, Substances Added to Food: AGAR (GELIDIUM SPP.)
Agar is a gelling agent from red seaweed and the usual vegetarian substitute for gelatin. It sets firmly, melts at a much higher temperature than gelatin, and is used in confectionery, desserts and canned meats.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | Permitted, with conditions | 54 food categories no numerical limit (good manufacturing practice) Show every food category
| Food Standards Code, Schedule 15, S15—5and 53 further provisionsretrieved 2026-10-17 |
| Canada | Permitted, with conditions | no numerical limit (good manufacturing practice) 10 permitted uses Show every permitted use
| Health Canadaand 9 further provisionsretrieved 2026-10-17 |
| European Union | Permitted, with conditions | 73 food categories highest permitted level 10,000 mg/l Show every food category
| Regulation (EC) No 1333/2008and 72 further provisionsretrieved 2026-10-18 |
| Great Britain | Permitted, with conditions | 70 food categories highest permitted level 10,000 mg/kg or mg/l Show every food category
| Assimilated Regulation (EC) No 1333/2008and 69 further provisionsretrieved 2026-10-18 |
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | No listing matchedThis site imports Part III of South Korea’s Standards and Specifications for Food Additives, matched by CAS number, then INS number, then exact name. Mixed preparations and sanitisers are not imported, and an entry whose identifiers disagreed with a page’s is held for review, so an empty row does not show that South Korea does not permit the substance. | ||
| United States | Permitted | 21 CFR 184.1115retrieved 2026-10-17 | |
| Codex Alimentarius | Permitted, with conditions | 23 food categories no numerical limit (good manufacturing practice) Show every food category and its notes
| CXS 192-1995 Table Three, INS 406and 23 further provisionsretrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| Australia and New Zealand | — | 54 GMP |
|---|---|---|
| Canada | — | 6 GMP, 4 no quantity stated |
| European Union | 10,000 mg/kg | 3 numeric limit, 46 several limits, 2 quantum satis, 22 no quantity stated |
| Great Britain | 10,000 mg/kg | 2 numeric limit, 68 quantum satis |
| United States | — | 1 no quantity stated |
| Codex Alimentarius | — | 24 GMP |
What it is made from
Extracted with hot water from red seaweeds, chiefly Gelidium and Gracilaria species, then frozen or pressed to remove water and dried. No animal material is involved.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
- CAS registry number
- 9002-18-0
The two specifications, compared
The European Union sets identity and purity by regulation; JECFA publishes its own through FAO, advisory until a country adopts it. Both documents are below in full. These are the parameters each puts a number on.
| Parameter | European Union | JECFA |
|---|---|---|
| Assay | stated in prose | stated in prose |
| Loss on drying | no more than 22 % | no more than 22 % |
| Lead | no more than 5 mg/kg | no more than 5 mg/kg |
| Arsenic | no more than 3 mg/kg | no more than 3 mg/kg |
| Mercury | no more than 1 mg/kg | — |
| Cadmium | no more than 1 mg/kg | — |
The two documents state the same figures for every parameter compared here.
How the EU defines it
The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.
E 406 Agar
Agar is a hydrophilic colloidal polysaccharide consisting mainly of galactose units with a regular alternation of L and D isomeric forms. These hexoses are alternately linked with alpha-1,3 and beta-1,4 bonds in the copolymer. On about every tenth D-galactopyranose unit one of the hydroxyl groups is esterified with sulphuric acid which is neutralised by calcium, magnesium, potassium or sodium. It is extracted from certain strains of marine algae of the families Gelidiaceae and Gracilariaceae and relevant red algae of the class Rhodophyceae
Synonyms: Gelose; Kanten, Bengal, Ceylon, Chinese or Japanese isinglass; Layor Carang
Assay, identification and purity criteria
Description and assay
- Einecs
- 232-658-1
- Assay
- The threshold gel concentration should not be higher than 0,25 %
- Description
- Agar is odourless or has a slight characteristic odour. Unground agar usually occurs in bundles consisting of thin, membranous, agglutinated strips, or in cut, flaked or granulated forms. It may be light yellowish-orange, yellowish-grey to pale yellow, or colourless. It is tough when damp, brittle when dry. Powdered agar is white to yellowish-white or pale yellow. When examined in water under a microscope, agar powder appears more transparent. In chloral hydrate solution, the powdered agar appears more transparent than in water, more or less granular, striated, angular and occasionally contains frustules of diatoms. Gel strength may be standardised by the addition of dextrose and maltodextrines or sucrose
Identification
- Solubility
- Insoluble in cold water; soluble in boiling water
Purity criteria
- Loss on drying
- Not more than 22 % (105 °C, 5 hours)
- Ash
- Not more than 6,5 % on the anhydrous basis determined at 550 °C
- Acid-insoluble ash (insoluble in approximately 3N Hydrochloric acid)
- Not more than 0,5 % determined at 550 °C on the anhydrous basis
- Insoluble matter (after stirring for 10 minutes in hot water)
- Not more than 1,0 %
- Starch
- Not detectable by the following method: to a 1 in 10 solution of the sample add a few drops of iodine solution. No blue colour is produced
- Gelatin and other proteins
- Dissolve about 1 g of agar in 100 ml of boiling water and allow to cool of about 50 °C. To 5 ml of the solution add 5 ml of trinitrophenol solution (1 g of anhydrous trinitrophenol/100 ml of hot water). No turbidity appears within 10 minutes
- Water absorption
- Place 5 g to agar in a 100 ml graduated cylinder, fill to the mark with water, mix and allow to stand at about 25 °C for 24 hours. Pour the contents of the cylinder through moistened glass wool, allowing the water to drain into a second 100 ml graduated cylinder. Not more than 75 ml of water is obtained
- Arsenic
- Not more than 3 mg/kg
- Lead
- Not more than 5 mg/kg
- Mercury
- Not more than 1 mg/kg
- Cadmium
- Not more than 1 mg/kg
- Microbiological criteria: Total plate count
- Not more than 5 000 colonies per gram
- Microbiological criteria: Yeast and moulds
- Not more than 300 colonies per gram
- Microbiological criteria: Escherichia coli
- Absent in 5 g
- Microbiological criteria: Salmonella spp.
- Absent in 5 g
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
INS 406 Agar
Agar is the dried hydrophilic, colloidal substance extracted from certain marine algae of the class Rhodophyceae. It is a polysaccharide, consisting primarily of D- and L-galactose units. About every tenth D-galactopyranose unit contains a sulfate ester group. Calcium, magnesium, potassium or sodium cations are also associated with the polysaccharide.
Synonyms: Agar-agar; gelose; Japan agar; Bengal, Ceylon, Chinese or Japanese isinglass; Layor Carang; INS No. 406 Bengal isinglass; Ceylon isinglass; Chinese isinglass; Japanese isinglass
Assay, identification and purity criteria
Description and assay
- Specification history
- Prepared at the 49th JECFA (1997), published in FNP 52 Add 5 superseding specifications prepared at the 44th JECFA (1995), published in FNP 52 Add 3 (1995). An ADI 'not limited' was establishe d at the 17th JECFA (1973)
- C.A.S. number
- 9002-18-0
- Assay
- Not higher than 0.25% for threshold gel concentration
- DESCRIPTION
- Odourless or has a slight characteristic odour. Unground agar usually occurs in bundles consisting of thin, membranous, agglutinated strips, or in cut, flaked, granulated or powdered forms. It may be light yellowish orange, yellowish grey to pale yellow, or colourless. It is tough when damp, brittle when dry. Powdered agar is white to yellowish white or pale yellow.
- FUNCTIONAL USES
- Thickener, stabilizer, emulsifier
- METHOD OF ASSAY
- Threshold gel concentration: Prepare serial dilutions of the sample with known solids content (0.15%, 0.20%, 0.25%, etc.) and place in tubes, 150 mm long by 16 mm internal diameter, stoppered at both ends. Cool for 1 h at 20-25 o . Allow cylinders of gel to slide from the tubes to a level surface. The lowest concentration of gel that resists gravity without rupture for 5-30 sec is the threshold concentration of the sample.
Identification
- Solubility
- Insoluble in cold water; soluble in boiling water
- Gel formation with water
- Prepare a 1.0% solution of the sample in boiling water in a flask and place the flask in water at 30 o for 15 min. A firm, resistant gel is formed. Place the flask in water at 70 o for 1 h, the gel is not molten. When heating the flask at higher temperature than 95 o , gel is liquefied to form a clear solution.
- Precipitate formation with ammonium sulfate solution
- A warm (40 o ) 0.5% solution of the sample gives a precipitate with half its volume of a warm (40 o ) 40% ammonium sulfate solution. This test distinguishes agar from alginates, gum arabic, gum ghatti, karaya gum, pectin and tragacanth.
- Precipitate formation with lead acetate solution
- A warm 0.5% solution of the sample gives a precipitate with one fifth its volume of basic lead acetate TS. This test distinguishes agar from methyl cellulose.
- Microscopy
- Place a few fragments of unground agar or some powder on a slide and add some drops of water or chloral hydrate TS. When examined under a microscope, agar in water appears granular and somewhat filamentous. In chloral hydrate TS, the powdered agar appears more transparent than in water.
Purity criteria
- Water absorption
- Place 5 g of the sample in a 100-ml graduated cylinder, fill to the mark with water, mix, and allow to stand at 25 o for 24 h. Pour the contents of the cylinder through moistened glass wool, allowing the water to drain into a second 100-ml graduated cylinder. Not more than 75 ml of water should be obtained.
- Loss on drying (Vol. 4)
- Not more than 22% after drying at 105 o until the difference between two weighings is less than 1 mg (about 5 h). Unground agar should be cut into pieces from 2 to 5 mm 2 before drying.
- Total ash (Vol. 4)
- Not more than 6.5% on the dried basis
- Acid-insoluble ash (Vol. 4)
- Not more than 0.5% on the dried basis
- Foreign insoluble matter
- Not more than 1% Boil 5 g of the sample with 500 ml of water and 12 ml of sulfuric acid under a reflux condenser for 2 h. Allow to cool and filter through a tared, fine, sintered glass crucible. Wash flask and filter with 50 ml of water, dry at 105 o to constant weight and weigh. Calculate as percentage.
- Starch and dextrins
- Not detectable To a warm (40 o ) 0.5% solution of the sample, add 2 drops of iodine TS. Where the drops fall, a red-violet colour appears. After mixing, the solution should be golden brown and not blue or reddish.
- Gelatin and other proteins
- Not detectable To a warm (40 o ) 0.5% solution of the sample add 1 volume of warm (40º) picric acid TS. No turbidity should appear within 10 min.
- Microbiological criteria (Vol. 4)
- Total plate count: Not more than 5,000 colonies per gram. Initially prepare a 10 -1 dilution by adding a 50 g sample to 450 ml of Butterfield’s phosphate buffered dilution water and homogenizing in a high speed blender. Yeasts and moulds: Not more than 500 colonies per gram Coliforms: Negative by test Salmonella: Negative by test
- Arsenic (Vol. 4)
- Not more than 3 mg/kg (Method II)
- Lead (Vol. 4)
- Not more than 5 mg/kg Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, “Instrumental Methods.”
Authorised in other additives, enzymes, flavourings and nutrients
These authorisations are not for use in food. They let this substance be used inside another additive, a food enzyme, a flavouring or a nutrient preparation — often as a carrier — and it reaches food only as part of that product. Annex III to Regulation (EC) No 1333/2008.
| Part | May be used in | Maximum level | Citation |
|---|---|---|---|
| Annex III, Part 1: Carriers in food additives | All food additives | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 1 |
| Annex III, Part 2: Food additives other than carriers in food additives | All food additive preparationsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" Note (1): Except enzymes authorised as food additives. | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 2, via Table 1 of Part 6 |
| Annex III, Part 3: Food additives including carriers in food enzymes | Food enzymesCan be used as a carrier: Yes Note (1): Including enzymes authorised as food additives. | In enzyme preparation: quantum satis In final food except beverages: quantum satis In beverages: quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 3 |
| Annex III, Part 4: Food additives including carriers in food flavourings | All flavouringsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 4, via Table 1 of Part 6 |
| Annex III, Part 5, Section A: Food additives added in nutrients except in nutrients intended to be used in foodstuffs listed in Part E, point 01.10, of Annex II and foodstuffs for infants and young children listed in Part E, point 13.1, of Annex II | All nutrientsCan be used as a carrier: Yes | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 5, Section A |
Every name it appears under
17 recorded names. A label may use any of them.
E number
- E406
INS number
- 406
CAS registry number
- 9002-18-0
As it appears in regulation
- Agar
- Agar-agar
Other names
- AGAR
- E 406
- Gelose
- Kanten, Bengal, Ceylon, Chinese or Japanese isinglass
- Layor Carang
Hazard reference values
| Type | Value | Population | Assessed by | Opinion |
|---|---|---|---|---|
| ADI | not limited | — | JECFA | 1973 |
Evidence and how bodies read it
What assessing bodies concluded
Each conclusion is paraphrased in the body's own framing, from the published opinion linked beneath it. Where bodies differ, both stand; this site does not adjudicate between them or add a view of its own.
EFSA 2016
The ANS Panel found no genotoxicity or carcinogenicity concern for agar (E 406) and concluded that no numerical ADI is needed and that there is no safety concern for the general population at the refined exposure assessment for reported uses. It noted that use-level data were received for only 7 of the 70 food categories in which agar is authorised.
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is E 406?
- E 406 is Agar. Agar is a gelling agent from red seaweed and the usual vegetarian substitute for gelatin. It sets firmly, melts at a much higher temperature than gelatin, and is used in confectionery, desserts and canned meats.
- What is Agar used for in food?
- It is listed as an emulsifier, a thickener and a stabiliser — the role Canada, Codex and the US assign it. This page shows every function each regulator records, with the citation for each.
- Where is Agar permitted?
- Of the seven regulators this site reads, Australia and New Zealand, Canada, the EU, Great Britain, the US and the Codex standard permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is Agar vegan?
- Yes. Agar is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- anz retrieved 2026-10-17
- ca retrieved 2026-10-17
- eu retrieved 2026-10-18
- gb retrieved 2026-10-18
- ecfr retrieved 2026-10-17
- gsfa retrieved 2026-09-07
- euspecs retrieved 2026-10-18
- jecfaspecs retrieved 2026-09-23
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