Carob bean gum
9000-40-2 · Algaroba gum · Beta-D-(1->4)mannan with some attached D-galactose molecules linked via alpha (1->6)bonds · CAROB BEAN GUM · Carob flour · E 410 · Galactomannan · Locust bean gum · E410 · 410 · Galactomannan · Locust (carob) bean gum · Locust bean gum · Brašno sjemenke rogača · Ceratoniju augļu sveķi · Farina di semi di carrube · Farine de graines de caroube · FARINHA DE SEMENTES DE ALFARROBA · Farinha de sementes de alfarroba (goma de alfarroba) · Fruktkärnmjöl · Goma garrofín · Gomma tal-ħarrub · gumi iz zrn rožičevca · GUMĂ DIN BOABE DE ROȘCOVE · Gumă din semințe de carruba · Jaanileivapuujahu · Johanneksenleipäpuujauhe · Johannesbroodpitmeel · Johannesbrødkernemel · Johannisbrotkernmehl · Karobová guma · Karubin · Locust bean gum · Mączka chleba świętojańskiego · Saldžiųjų ceratonijų derva · Szentjánoskenyérliszt · Κόμμι χαρουπιών · Гума локуст бийнE number E410INS 410CAS 9000-40-2
Listed as an emulsifier, a thickener and a stabiliser by Canada, Codex and the US.
Listed as emulsifiers (CA, US, Codex) · thickeners (CA, US, Codex) · stabilisers (CA, US, Codex) · gelling agents (Codex) · flavour enhancers (US) · flavouring agents and adjuvants (US) · texturisers (US) · solvents and vehicles (US)
- Canada: Emulsifying agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Emulsifying, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Emulsifier or emulsifier salt — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- Codex Alimentarius: Emulsifier — CXS 192-1995 Table One, INS 410
- Canada: Emulsifying, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Stabilizer or thickener — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- United States: Stabilizers and thickeners — 21 CFR 184.1343, citing 21 CFR 170.3(o)(28)
- Codex Alimentarius: Thickener — CXS 192-1995 Table One, INS 410
- Canada: Emulsifying, stabilizing or thickening agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- Canada: Stabilizing agent — Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)
- United States: Stabilizer or thickener — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- United States: Stabilizers and thickeners — 21 CFR 184.1343, citing 21 CFR 170.3(o)(28)
- Codex Alimentarius: Stabilizer — CXS 192-1995 Table One, INS 410
- Codex Alimentarius: Gelling agent — CXS 192-1995 Table One, INS 410
- United States: Flavor enhancer — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- United States: Flavoring agent or adjuvant — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- United States: Texturizer — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
- United States: Solvent or vehicle — FDA, Substances Added to Food: LOCUST (CAROB) BEAN GUM
Carob bean gum, also called locust bean gum, is a thickener from the seed of the carob tree. It is used in ice cream, cream cheese and sauces, and works particularly well combined with xanthan or carrageenan.
Where it is and isn’t permitted
| Jurisdiction | Status | Scope and limits | Citation |
|---|---|---|---|
| Australia and New Zealand | Permitted, with conditions | 57 food categories highest permitted level 10,000 mg/L Show every food category
| Food Standards Code, Schedule 15, S15—5and 56 further provisionsretrieved 2026-10-17 |
| Canada | Permitted, with conditions | no numerical limit (good manufacturing practice) 15 permitted uses Show every permitted use
| Health Canada, List of Permitted Emulsifying, Gelling, Stabilizing or Thickening Agents (List 4)and 14 further provisionsretrieved 2026-10-17 |
| European Union | Permitted, with conditions | 81 food categories highest permitted level 10,000 mg/l Show every food category
| Regulation (EC) No 1333/2008and 80 further provisionsretrieved 2026-10-18 |
| Great Britain | Permitted, with conditions | 78 food categories highest permitted level 10,000 mg/kg or mg/l Show every food category
| Assimilated Regulation (EC) No 1333/2008and 77 further provisionsretrieved 2026-10-18 |
| Japan | No listing matchedThis site imports only Japan’s list of designated additives (Appended Table 1 to the Regulations for Enforcement of the Food Sanitation Act), matched by English name. Existing additives, natural flavouring agents and ordinary foods used as additives are permitted outside that list and are not imported, so an empty row does not show that Japan does not permit the substance. | ||
| South Korea | Permitted, with conditions | Designated food additive: Standards and Specifications for Food Additives, Part III, 1. …Designated food additive: Standards and Specifications for Food Additives, Part III, 1. 일반식품첨가물 (general food additives), 로커스트콩검 (“Locust Bean Gum”, the English the Code prints beside it). Main use (주용도) of 로커스트콩검: 증점제, 안정제, 유화제. Standard for use (사용기준) of 로커스트콩검: (1) [to be used under the general standard for use in II. 2. 1), the minimum amount that achieves the technical effect, with no limit set for this additive in particular] “II. 2. 1)의 규정에 따라 사용하여야 한다.” The Korean is the standard as published. There is no official English translation of the current Code (the English version on MFDS’s website is the 2020 edition and has no legal effect); the English in square brackets is this site’s description of what each sentence does, not a translation of the foods or the levels. Compositional specifications (Part IV) are not imported, nor are the general standards in Part II, which apply to every additive. | Standards and Specifications for Food Additives (식품첨가물의 기준 및 규격), MFDS Notification, Part III (Standards for use, by item)retrieved 2026-10-18 |
| United States | Permitted | 21 CFR 184.1343retrieved 2026-10-17 | |
| Codex Alimentarius | Permitted, with conditions | 22 food categories highest permitted level 2,000 mg/kg Show every food category and its notes
| CXS 192-1995 Table Three, INS 410and 22 further provisionsretrieved 2026-09-07 |
How much is permitted
A listing usually limits the quantity as well as the food. Only one of the forms that takes is a figure: quantum satis and good manufacturing practice permit the amount the technological effect requires and no more, which is a limit rather than the absence of one. Figures below are the range across every food category this page is listed in, and categories differ between regulators — see how much is permitted.
| Australia and New Zealand | 1,000 mg/kg – 10,000 mg/kg | 3 numeric limit, 54 GMP |
|---|---|---|
| Canada | — | 5 GMP, 10 no quantity stated |
| European Union | 1,000 mg/kg – 10,000 mg/kg | 8 numeric limit, 46 several limits, 5 quantum satis, 22 no quantity stated |
| Great Britain | 1,000 mg/kg – 10,000 mg/kg | 6 numeric limit, 1 several limits, 71 quantum satis |
| South Korea | — | 1 no quantity stated |
| United States | — | 1 no quantity stated |
| Codex Alimentarius | 1,000 mg/kg – 2,000 mg/kg | 4 numeric limit, 19 GMP |
What it is made from
Milled from the endosperm of the seed of Ceratonia siliqua, the carob tree of the Mediterranean. The process is mechanical separation and grinding.
- Vegan
- Vegetarian
Compare other additives on the dietary indexes.
- CAS registry number
- 9000-40-2
The two specifications, compared
The European Union sets identity and purity by regulation; JECFA publishes its own through FAO, advisory until a country adopts it. Both documents are below in full. These are the parameters each puts a number on.
| Parameter | European Union | JECFA |
|---|---|---|
| Assay | no less than 75 % | — |
| Loss on drying | no more than 15 % | no more than 14 % |
| Lead | no more than 0.4 mg/kg | no more than 2 mg/kg |
| Arsenic | no more than 0.1 mg/kg | no more than 3 mg/kg |
| Mercury | no more than 0.1 mg/kg | — |
| Cadmium | no more than 0.1 mg/kg | — |
3 parameters differ between the two documents. A difference is not a disagreement about safety: the two are revised on their own cycles, and an assay or a drying loss can be measured against a different preparation of the substance, which is why only the contaminant ceilings are marked. Within the EU the EU's figure is the one that binds.
How the EU defines it
The legal specification an additive must meet to be sold as this E number in the EU — what it is, how it may be made, and the purity it must reach. From Commission Regulation (EU) No 231/2012.
E 410 Locust bean gum
Locust bean gum is the ground endosperm of the seeds of the strainsof carob tree, Cerationia siliqua (L.) Taub. (family Leguminosae). The seeds are dehusked by treating the kernels with dilute sulfuric acid or with thermal mechanical treatments, elimination of the germ, followed by milling and screening of the endosperm to obtain native locust bean gum. Locust bean gum consists mainly of a high molecular weight hydrocolloidal polysaccharide, composed of galactopyranose and mannopyranose units combined through glycosidic linkages, which may be described chemically as galactomannan.
Synonyms: Carob bean gum; Algaroba gum
Assay, identification and purity criteria
Description and assay
- Einecs
- 232-541-5
- CAS number
- 9000-40-2
- Molecular weight
- 50 000 –3 000 000
- Assay
- Galactomannan content not less than 75 %
- Description
- White to yellowish-white, nearly odourless powder
Identification
- Test for galactose
- Passes test
- Test for mannose
- Passes test
- Microscopic examination
- Place some ground sample in an aqueous solution containing 0,5 % iodine and 1 % potassium iodide on a glass slide and examine under microscope. Locust bean gum contains long stretched tubiform cells, separated or slightly interspaced. Their brown contents are much less regularly formed than in guar gum. Guar gum shows close groups of round to pear-shaped cells. Their contents are yellow to brown
- Solubility
- Fully dispersible in hot water, insoluble in ethanol
Purity criteria
- Loss on drying
- Not more than 15 % (105 °C, 5 hours)
- Ash
- Not more than 1,2 % determined at 800 °C
- Protein (N × 6,25)
- Not more than 7 %
- Acid-insoluble matter
- Not more than 4 %
- Starch
- Not detectable by the following method: to a 1 in 10 dispersion of the sample add a few drops of iodine solution. No blue colour is produced.
- Arsenic
- Not more than 0,1 mg/kg
- Lead
- Not more than 0,4 mg/kg
- Mercury
- Not more than 0,1 mg/kg
- Cadmium
- Not more than 0,1 mg/kg
- Ethanol and propan-2-ol
- Not more than 1 %, single or in combination
- Microbiological criteria: Total plate count
- Not more than 5 000 cfu/g
- Microbiological criteria: Yeast and moulds
- Not more than 500 cfu/g
- Microbiological criteria: Enterobacteriaceae
- Absent in 10 g (only if added to dried infant formulae, dried foods for special medical purposes intended for infants below 6 months of age and dried follow-on formulae)
- Microbiological criteria: Escherichia coli
- Absent in 1 g
- Microbiological criteria: Salmonella spp.
- Absent in 25 g
- Microbiological criteria: Cronobacter spp. (Enterobacter sakazakii)
- Absent in 10 g (only if added to dried infant formulae and dried foods for special medical purposes intended for infants below 6 months of age)
How JECFA defines it
The international specification for identity and purity, prepared by the Joint FAO/WHO Expert Committee on Food Additives and published by FAO. It is a different document from any above, written by a different body and revised on its own cycle; it is advisory, and a country adopts it or does not.
INS 410 Carob Bean Gum
Carob bean gum, also known as locust bean gum, is a galactomannan polysaccharide obtained from the seeds of Ceratonia siliqua (L.) Taub. (Fam. Leguminosae ). The ground endosperm of the seeds consists mainly of high molecular weight (approximately 50,000-3,000,000) polysaccharides composed of galactomannans with a mannose:galactose ratio of about 4:1. The seeds are dehusked by treating the seeds with dilute sulfuric acid or with thermal mechanical treatments, elimination of the germ followed by milling and screening of the endosperm to obtain native carob bean gum. The gum may be washed with ethanol or isopropanol to control the microbiological load (washed carob bean gum).
Synonyms: Locust bean gum, INS No. 410 Locust bean gum
Assay, identification and purity criteria
Description and assay
- Specification history
- Prepared at the 82 nd JECFA and published in FAO JECFA Monographs 19 (2016) superseding specifications prepared at the 69 th JECFA (2008) and published in FAO JECFA Monographs 5 (2008), superseding tentative specifications prepared at the 67 th JECFA (2006) and published in FAO JECFA Monographs 3 (2006). An ADI "not specified" was established at the 25 th JECFA (1981).
- C.A.S. number
- 9000-40-2
- DESCRIPTION
- White to yellowish white, nearly odourless powder
- FUNCTIONAL USES
- Thickener, stabilizer, emulsifier, gelling agent
Identification
- Solubility (Vol. 4)
- Insoluble in ethanol
- Gel formation
- Add small amounts of sodium borate TS to an aqueous dispersion of the sample; a gel is formed.
- Viscosity
- Transfer 2 g of the sample into a 400-ml beaker and moisten thoroughly with about 4 ml of isopropanol. Add 200 ml of water with vigorous stirring until the gum is completely and uniformly dispersed. An opalescent, slightly viscous solution is formed. Transfer 100 ml of this solution into another 400-ml beaker. Heat the mixture in a boiling water bath for about 10 min and cool to room temperature. There is an appreciable increase in viscosity (differentiating carob bean gums from guar gums).
- Gum constituents (Vol. 4)
- Proceed as directed under Gum Constituents Identification using 100 mg of the sample instead of 200 mg and 1 to 10 μl of the hydrolysate instead of 1 to 5 μl. Use galactose and mannose as reference standards. These constituents should be present.
- Microscopic examination
- Disperse a sample of the gum in an aqueous solution containing 0.5% iodine and 1% potassium iodide on a glass slide and examine under a microscope. Carob bean gum contains long stretched tubiform cells, separated or slightly interspaced. Their brown contents are much less regularly formed than in Guar gum.
Purity criteria
- Loss on drying (Vol. 4)
- Not more than 14% (105º, 5 h)
- Total ash ( Vol. 4)
- Not more than 1.2% (800º, 3-4 h)
- Acid-insoluble matter (Vol. 4)
- Not more than 4.0%
- Protein (Vol. 4)
- Not more than 7.0% Proceed as directed under Nitrogen Determination (Kjeldahl Method) in Volume 4 (under “General Methods, Inorganic components”). The percentage of nitrogen determined multiplied by 6.25 gives the percentage of protein in the sample.
- Starch
- To a 1 in 10 dispersion of the sample add a few drops of iodine TS; no blue colour is produced
- Residual solvents
- Not more than 1% of ethanol or isopropanol, singly or in combination See description under TESTS
- Lead (Vol. 4)
- Not more than 2 mg/kg Determine using method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”). Not more than 0.5 mg/kg for use in infant formula and formula for special medical purposes intended for infants .
- Arsenic (Vol. 4) (Vol. 4)
- Not more than 3 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”). Initially prepare a 10 -1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate-buffered dilution water and homogenising the mixture in a high-speed blender. Total (aerobic) plate count: Not more than 5,000 CFU/g E. coli: Negative in 1g
- TESTS
- Salmonella: Negative in 25 g Yeasts and moulds: Not more than 500 CFU/g
- Test: Residual solvents
- Determine residual solvents using headspace gas chromatography (Method I) Internal standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl of 3-methyl-2-pentanone through the septum and reweigh to within 0.01 mg. Standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl ethanol and weigh to within 0.01mg. Inject 15 μl isopropanol through the septum and reweigh the vial. Blank solution: Add 5.0 ml of water and pipette 1.0 ml of the internal standard solution into a headspace vial. Seal the vial and mix the contents using a vortex mixer. Calibration solution: Add 4.0 ml of water into the headspace vial. Pipette 1.0 ml each of the internal standard solution and the standard solution. Seal the vial and mix the contents using a vortex mixer. Preparation of sample: Accurately weigh 0.500+0.001 g of sample in a small weighing boat. Pipette 5 ml of water and 1 ml internal standard solution into a headspace vial. Add the sample carefully to prevent clumping of sample at the bottom of the vial. Seal the vial and mix the contents using a vortex mixer. Do not shake the sample vial. Follow the procedure described in Vol. 4.
INS 410 Carob Bean Gum (Clarified)
Carob bean gum, also known as locust bean gum, is a galactomannan polysaccharide obtained from the seeds of Ceratonia siliqua (L.) Taub. (Fam. Leguminosae ). The ground endosperm consists mainly of high molecular weight (approximately 50,000-3,000,000) polysaccharides composed of galactomannans with a mannose:galactose ratio of about 4:1. The seeds are dehusked by treating the seeds with dilute sulfuric acid or with thermal mechanical treatments, elimination of the germ, followed by milling and screening of the endosperm to obtain native carob bean gum. The gum is clarified by dispersing in hot water, filtration and precipitation with ethanol or isopropanol, filtering, drying and milling. The clarified carob bean gum does not contain cell wall materials. Clarified carob bean gum in the market is normally standardized with sugars for viscosity and reactivity.
Synonyms: Locust bean gum clarified, INS No. 410 Locust bean gum clarified
Assay, identification and purity criteria
Description and assay
- Specification history
- Page 1 of 7 Prepared at the 82 nd JECFA and published in JECFA Monograph 19 (2016) superseding specifications prepared at the 69 th JECFA (2008) and published in FAO JECFA Monographs 5 (2008), superseding tentative specifications prepared at the 67th JECFA (2006) and published in FAO JECFA Monographs 3 (2006). An ADI "not specified" was established at the 25th JECFA (1981) for carob bean gum.
- C.A.S. number
- 9000-40-2
- DESCRIPTION
- White to yellowish white, nearly odourless powder
- FUNCTIONAL USES
- Stabilizer, thickener, emulsifier, gelling agent
- METHOD OF ASSAY
- The difference between 100 and the sum of the percent Loss on Drying, Total Ash, Acid-Insoluble Matter, Protein and Crude Fat represents the percent Galactomannans .
Identification
- Solubility (Vol. 4)
- Insoluble in ethanol
- Gel formation
- Add small amounts of sodium borate TS to an aqueous dispersion of the sample; a gel is formed.
- Viscosity
- Transfer 2 g of the sample into a 400-ml beaker and moisten thoroughly with about 4 ml of isopropanol. Add 200 ml of water with vigorous stirring until the gum is completely and uniformly dissolved. An opalescent, slightly Page 2 of 7 viscous solution is formed. Transfer 100 ml of this solution into another 400-ml beaker. Heat the mixture in a boiling water bath for about 10 min and cool to room temperature. There is an appreciable increase in viscosity (differentiating carob bean gums from guar gums).
- Gum constituents (Vol. 4)
- Proceed as directed under Gum Constituents Identification using 100 mg of the sample instead of 200 mg and 1 to 10 μl of the hydrolysate instead of 1 to 5 μl. Use galactose and mannose as reference standards. These constituents should be present.
- Gel formation with borate
- Add sufficient amounts of sodium borate TS to an aqueous dispersion of the sample sufficient to raise the pH to above 9; a gel is formed. Page 5 of 7
- Gel formation with xanthan gum
- Passes test See description under tests
- Gum constituents (Vol. 4)
- Proceed as directed under Gum Constituents Identification (Vol. 4) using 100 mg of sample instead of 200 mg and 1-10 μl of the hydrolysate instead of 1-5 μl. Use galactose and mannose as reference standards. These constituents should be present.
- Viscosity
- Less than 500 mPas (25º, 2h) (1% solution) See description under TESTS
- pH (Vol. 4)
- 5.5-8.0 (1%)
- Gel formation with xanthan gum
- Weigh 1.5 g of the sample and 1.5 g of xanthan gum and blend them. Add this blend with (rapid stirring) into 300 ml water at 80º in a 400 ml Page 6 of 7 beaker. Stir until the mixture is dissolved and continue stirring for an extra 30 min after dissolution (maintain the temperature above 60º during the stirring process). Discontinue stirring and allow the mixture to cool at room temperature for at least 2 h. A firm, viscoelastic gel forms after the temperature drops below 40º, but no such gel forms in a 1% control solution of cassia gum or xanthan gum alone prepared in a similar manner.
- Viscosity
- Weigh 5 g of the sample in a plastic dish and 495 g of distilled water at 20º in a 1000 ml beaker. Add a magnetic bar and place the beaker on the agitation plate. Adjust the speed of agitation to 750 rpm. Introduce quickly the 5 g of sample in the water and cover the beaker with a watch glass. Keep the temperature at 90º for 15 min. Cool the solution at 25º (the cooling must be ±1.5º) in a water bath and measure the viscosity after 2 h at 25º using a RVT Brookfield Spindle 1, speed 20 rpm. Repeat the procedure with a sample of 5 g of carob (locust) bean gum. (Note: The viscosity of the cassia gum (150 - 500 mPas) must be less than 50% that of carob bean gum (2000 - 3500 mPas))
Purity criteria
- Loss on drying (Vol. 4)
- Not more than 14% (105º, 5 h)
- Total ash (Vol. 4)
- Not more than 1.2% (800º, 3-4 h)
- Acid-insoluble matter (Vol. 4)
- Not more than 3.5%
- Protein (Vol. 4)
- Not more than 1.0% Proceed as directed under Nitrogen Determination (Kjeldahl Method) in Volume 4 (under “General Methods, Inorganic components”). The percentage of nitrogen determined multiplied by 6.25 gives the percentage of protein in the sample.
- Starch
- To a 1 in 10 solution of the sample add a few drops of iodine TS; no blue colour is produced
- Residual solvents
- Not more than 1% of ethanol or isopropanol, singly or in combination See description under TESTS
- Lead (Vol. 4)
- Not more than 2 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”). Not more than 0.5 mg/kg for use in infant formula and formula for special medical purposes intended for infants.
- Arsenic (Vol. 4)
- Not more than 3 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based
- Microbiological criteria (Vol. 4)
- on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”). Initially prepare a 10 -1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate-buffered dilution water and homogenising the mixture in a high-speed blender. Total (aerobic) plate count: Not more than 5,000 CFU/g E. coli: Negative in 1 g Salmonella : Negative in 25 g Yeasts and moulds: Not more than 500 CFU/g Page 3 of 7
- Test: Residual solvents
- Determine residual solvents using headspace gas chromatography (Method I) Internal standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl of 3-methyl-2-pentanone through the septum and reweigh to within 0.01 mg. Standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl ethanol and weigh to within 0.01mg. Inject 15 μl isopropanol through the septum and reweigh the vial. Blank solution: Add 5.0 ml of water and pipette 1.0 ml of the internal standard solution into a headspace vial. Seal the vial and mix the contents using a vortex mixer. Calibration solution: Add 4.0 ml of water into the headspace vial. Pipette 1.0 ml each of the internal standard solution and the standard solution. Seal the vial and mix the contents using a vortex mixer. Preparation of sample: Accurately weigh 0.500+0.001 g of sample in a small weighing boat. Pipette 5 ml of water and 1 ml internal standard solution into a headspace vial. Add the sample carefully to prevent clumping of sample at the bottom of the vial. Seal the vial and mix the contents using a vortex mixer. Do not shake the sample vial. Follow the procedure described in Vol. 4. Page 4 of 7 CASSIA GUM (TENTATIVE) Revised and made tentative at the 82nd JECFA (2016) and published in FAO JECFA Monograph 19 (2016), superseding specifications prepared at the 73rd JECFA (2010) and published in FAO JECFA Monographs 10 (2010). An ADI “not specified” was established at the 71 st JECFA (2009). Information required: • Suitable method for the determination of anthraquinones, including: the use of standard (reference) materials the extraction efficiency of the initial steps the recovery of the analytes in question performance data for the method results of analysis by an accredited laboratory of a minimum of five batches of material The tentative specifications will be withdrawn unless the requested information is submitted before 31 st December 2017.
- Test: SYNONYMS
- INS 427
- Test: DEFINITION
- Primarily the ground purified endosperm of the seeds of Cassia tora and Cassia obtusifolia , (Fam. Leguminsae ) containing less than 0.05% of Cassia occidentalis. It consists mainly of high molecular weight (approximately 200,000-300,000) polysaccharides composed of galactomannans; the mannose:galactose ratio is about 5:1. The structural formula for cassia gum galactomannan is given below. The seeds are dehusked and degermed by thermal mechanical treatment followed by milling and screening of the endosperm. The ground endosperm is further purified by extraction with isopropanol.
- Test: Assay
- Not less than 75% of galactomannans
- Test: DESCRIPTION
- Pale yellow to off-white, odourless free-flowing powder
- Test: FUNCTIONAL USES Solubility
- Thickener, emulsifier, foam stabilizer, moisture retention agent and texturizing agent. Insoluble in ethanol Disperses well in cold water forming colloidal solutions.
- Loss on drying (Vol. 4)
- Not more than 12% (105º , 5 h)
- Total ash (Vol. 4)
- Not more than 1.2%
- Acid-insoluble matter (Vol. 4)
- Not more than 2.0%
- Protein (Vol. 4)
- Not more than 7.0% Proceed as directed under Nitrogen Determination (Kjeldahl Method; Vol. 4). The percent of nitrogen in the sample multiplied by 6.25 gives the percent of protein in the sample.
- Crude fat
- Not more than 1% See description under TESTS
- Starch
- To a 1 in 10 dispersion of the sample add a few drops of iodine TS; no blue colour is produced.
- Anthraquinones
- Not more than 0.5 mg/kg See description under TESTS
- Residual solvents (Vol. 4)
- Isopropanol: Not more than 1.0% See description under Tests
- Lead (Vol. 4)
- Not more than 1 mg/kg Determine using a method appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4 (under "General Methods, Metallic Impurities").
- Microbiological criteria (Vol. 4)
- Total plate count: Not more than 5,000 cfu/g Yeast and mould: Not more than 100 cfu/g E. coli : Negative in 1 g Salmonella : Negative in 25 g
- Test: Residual solvents
- Determine residual solvents using headspace gas chromatography (Vol. 4; Method I) Internal standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl of 3-methyl-2-pentanone through the septum and reweigh to within 0.01 mg. Standard solution: Add 50.0 ml water to a 50 ml vial and seal. Accurately weigh and inject 15 μl ethanol and weigh to within 0.01mg. Inject 15 μl isopropanol through the septum and reweigh the vial. Blank solution: Add 5.0 ml of water and pipette 1.0 ml of the internal standard solution into a headspace vial. Seal the vial and mix the contents using a vortex mixer. Calibration solution: Add 4.0 ml of water into the headspace vial. Pipette 1.0 ml each of the internal standard solution and the standard solution. Seal the vial and mix the contents using a vortex mixer. Preparation of sample: Accurately weigh 0.500 ± 0.001 g of sample in a small weighing boat. Pipette 5 ml of water and 1 ml internal standard solution into a headspace vial. Add the sample carefully to prevent clumping of sample at the bottom of the vial. Seal the vial and mix the contents using a vortex mixer. Do not shake the sample vial. Follow the procedure described in Vol. 4.
- Test: Crude fat
- Apparatus The apparatus consisting of a Butt-type extractor, as shown below, having a standard-taper 34/45 female joint at the upper end, to which is attached a Friedrichs- or Hopkins-type condenser, and a 24/40 male joint at the lower end, to which is attached a 125-ml Erlenmeyer flask. Page 7 of 7 Procedure Transfer about 10 g of the sample, previously ground to 20-mesh or finer and accurately weighed, to a 15-cm filter paper, roll the paper tightly around the sample, and place it in a suitable extraction shell. Plug the top of the shell with cotton previously extracted with hexane, and place the shell in the extractor. Attach the extractor to a dry 125-ml Erlenmeyer flask containing about 50 ml of hexane and to a water-cooled condenser, apply heat to the flask to produce 150 to 200 drops of condensed solvent per min, and extract for 16 h. Disconnect the flask, and filter the extract to remove any insoluble residue. Rinse the flask and filter with a few ml of hexane, combine the washings and filtrate in a tared flask, and evaporate on a steam bath until no odor of solvent remains. Dry in a vacuum for 1 h at 100°, cool in a desiccator, and weigh. Butt-Type Extractor for fat determination. NOTE: The method for crude fat is referenced from the Food Chemicals Codex, 6th Edition, 2008, p. 1163. Reprinted with permission from the US Pharmacopeia, 12601 Twinbrook Parkway, Rockville, MD USA 20852.
- Test: Anthraquinones
- A validated method for determination of anthraquinones at the specified maximum level is requested
Authorised in other additives, enzymes, flavourings and nutrients
These authorisations are not for use in food. They let this substance be used inside another additive, a food enzyme, a flavouring or a nutrient preparation — often as a carrier — and it reaches food only as part of that product. Annex III to Regulation (EC) No 1333/2008.
| Part | May be used in | Maximum level | Citation |
|---|---|---|---|
| Annex III, Part 1: Carriers in food additives | All food additives | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 1 |
| Annex III, Part 2: Food additives other than carriers in food additives | All food additive preparationsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" Note (1): Except enzymes authorised as food additives. | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 2, via Table 1 of Part 6 |
| Annex III, Part 3: Food additives including carriers in food enzymes | Food enzymesCan be used as a carrier: Yes Note (1): Including enzymes authorised as food additives. | In enzyme preparation: quantum satis In final food except beverages: quantum satis In beverages: quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 3 |
| Annex III, Part 4: Food additives including carriers in food flavourings | All flavouringsAuthorised as a member of Table 1 of Part 6, listed as "Table 1" | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 4, via Table 1 of Part 6 |
| Annex III, Part 5, Section A: Food additives added in nutrients except in nutrients intended to be used in foodstuffs listed in Part E, point 01.10, of Annex II and foodstuffs for infants and young children listed in Part E, point 13.1, of Annex II | All nutrientsCan be used as a carrier: Yes | quantum satis | Regulation (EC) No 1333/2008, Annex III, Part 5, Section A |
Every name it appears under
38 recorded names. A label may use any of them.
E number
- E410
INS number
- 410
CAS registry number
- 9000-40-2
As it appears in regulation
- Galactomannan
- Locust (carob) bean gum
- Locust bean gum
Other names
- Algaroba gum
- Beta-D-(1->4)mannan with some attached D-galactose molecules linked via alpha (1->6)bonds
- CAROB BEAN GUM
- Carob flour
- E 410
- Galactomannan
- Locust bean gum
The EU re-evaluation programme
Every additive authorised in the EU before 20 January 2009 was put back through EFSA by Commission Regulation (EU) No 257/2010, and this one is named in it. What the Regulation required is below; whether the opinion has since been published is not something this site records.
- To be evaluated by 31 December 2016. Named in Annex II, Part II, point 2 as “Locust bean gum”, among the additives given priority within its class.
Hazard reference values
EFSA and JECFA have both assessed Carob bean gum and their conclusions are not two comparable figures — EFSA records not allocated, JECFA not specified. An acceptable daily intake is a reference value set by a committee, not a threshold above which harm begins. “Not specified”, “not limited” and “not allocated” are conclusions, not missing data, and they are shown in the words each body used rather than reduced to a number. See where the two committees differ.
| Type | Value | Population | Assessed by | Opinion |
|---|---|---|---|---|
| ADI | not specified | — | JECFA | 2025 |
| ADI | not allocated | infants | EFSA FAF | 2023 |
Evidence and how bodies read it
What assessing bodies concluded
Each conclusion is paraphrased in the body's own framing, from the published opinion linked beneath it. Where bodies differ, both stand; this site does not adjudicate between them or add a view of its own.
EFSA 2023
In a follow-up covering infants, the FAF Panel identified a reference point of 1,400 mg/kg bw/day for locust bean gum (E 410), based on reduced blood zinc in piglets, and used a margin of exposure approach for special medical foods. A margin above 1 was obtained for some infant scenarios and all toddler exposure levels, and it concluded that technical data support amending the specifications.
EFSA 2017
The ANS Panel concluded that no numerical ADI is needed for locust bean gum (E 410) and that there is no safety concern for the general population at the refined exposure assessment. It concluded that the data did not allow an adequate assessment of its use in foods for special medical purposes for infants and young children.
Common questions
Assembled from what is above, in the words the questions are usually asked in. Each answer is the same statement the relevant section makes, with the same citations behind it.
- What is E 410?
- E 410 is Carob bean gum. Carob bean gum, also called locust bean gum, is a thickener from the seed of the carob tree. It is used in ice cream, cream cheese and sauces, and works particularly well combined with xanthan or carrageenan.
- What is Carob bean gum used for in food?
- It is listed as an emulsifier, a thickener and a stabiliser — the role Canada, Codex and the US assign it. This page shows every function each regulator records, with the citation for each.
- Where is Carob bean gum permitted?
- Of the seven regulators this site reads, Australia and New Zealand, Canada, the EU, Great Britain, South Korea, the US and the Codex standard permit it. Each permission is for named foods at named levels, not a blanket one; the table on this page gives them with citations.
- Is Carob bean gum vegan?
- Yes. Carob bean gum is not derived from animals, and is suitable for vegetarians. This page says what it is made from and how.
Sources for this page
- anz retrieved 2026-10-17
- ca retrieved 2026-10-17
- eu retrieved 2026-10-18
- gb retrieved 2026-10-18
- kr retrieved 2026-10-18
- ecfr retrieved 2026-10-17
- gsfa retrieved 2026-09-07
- euspecs retrieved 2026-10-18
- jecfaspecs retrieved 2026-09-23
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